TY - JOUR
T1 - Presence of neural progenitors in spontaneous canine gliomas: A histopathological and immunohistochemical study of 20 cases
AU - Fernández, Francisco
AU - Deviers, Alexandra
AU - Dally, Claire
AU - Mogicato, Giovanni
AU - Delverdier, Maxence
AU - Cauzinille, Laurent
AU - Gnirs, Kirsten
AU - Añor, Sònia
AU - de la Fuente, Cristian
AU - Fondevila, Dolors
AU - Pumarola, Martí
N1 - Funding Information:
We gratefully acknowledge Ester Blasco and Lola Pérez for technical support, and Royal Canin Company, especially Isabelle Mougeot , for providing financial support for this study. Preliminary results were presented as an abstract at the 25th Symposium of the Spanish Society of Veterinary Pathology (SEAPV), Toledo, Spain, 19–21 June 2013, and as an oral communication at the Second Joint European Congress of the European Society of Veterinary Pathology, European Society of Toxicologic Pathology and the European College of Veterinary Pathologists, Berlin, Germany, 27–30 August 2014.
Publisher Copyright:
© 2016 Elsevier Ltd.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2016/3/1
Y1 - 2016/3/1
N2 - © 2016 Elsevier Ltd. Gliomas are the most common primary brain tumours in humans and are associated with a poor prognosis. An accurate animal model of human glioma tumorigenesis is needed to test new treatment strategies. Dogs represent a promising model because they develop spontaneous diffusely-infiltrating gliomas. This study investigated whether spontaneous canine gliomas contain cancer stem cells previously identified in all grades of human gliomas. Twenty spontaneous cases of canine gliomas were graded according to the human WHO classification. The expression of different markers of lineage differentiation was evaluated with immunohistochemistry as follows: nestin and CD133 for neural stem cells, doublecortin for neuronal progenitor cells, Olig2 for glial progenitor cells, glial fibrillary acidic protein, vimentin and S-100 for mature glial cells, and NeuN and βIII-tubulin for mature neurons. Gliomas were characterised as follows: five grade II (oligodendrogliomas); nine grade III (seven anaplastic oligodendrogliomas, one anaplastic astrocytoma, one anaplastic oligoastrocytoma); six grade IV (glioblastomas).Immunohistochemical evaluation revealed that (1) nestin and CD133 were expressed in all grades of gliomas with a higher proportion of positive cells in high-grade gliomas; (2) the expression of S-100 protein and Olig2 did not differ substantially between astrocytic and oligodendroglial tumours, and (3) all gliomas were negative for mature neuron markers. The results demonstrated the presence of undifferentiated neural progenitors in all grades of spontaneous canine gliomas, confirming the relevance of this animal model for further studies on cancer stem cells.
AB - © 2016 Elsevier Ltd. Gliomas are the most common primary brain tumours in humans and are associated with a poor prognosis. An accurate animal model of human glioma tumorigenesis is needed to test new treatment strategies. Dogs represent a promising model because they develop spontaneous diffusely-infiltrating gliomas. This study investigated whether spontaneous canine gliomas contain cancer stem cells previously identified in all grades of human gliomas. Twenty spontaneous cases of canine gliomas were graded according to the human WHO classification. The expression of different markers of lineage differentiation was evaluated with immunohistochemistry as follows: nestin and CD133 for neural stem cells, doublecortin for neuronal progenitor cells, Olig2 for glial progenitor cells, glial fibrillary acidic protein, vimentin and S-100 for mature glial cells, and NeuN and βIII-tubulin for mature neurons. Gliomas were characterised as follows: five grade II (oligodendrogliomas); nine grade III (seven anaplastic oligodendrogliomas, one anaplastic astrocytoma, one anaplastic oligoastrocytoma); six grade IV (glioblastomas).Immunohistochemical evaluation revealed that (1) nestin and CD133 were expressed in all grades of gliomas with a higher proportion of positive cells in high-grade gliomas; (2) the expression of S-100 protein and Olig2 did not differ substantially between astrocytic and oligodendroglial tumours, and (3) all gliomas were negative for mature neuron markers. The results demonstrated the presence of undifferentiated neural progenitors in all grades of spontaneous canine gliomas, confirming the relevance of this animal model for further studies on cancer stem cells.
KW - Cancer stem cells
KW - Canine
KW - Glioma
KW - Histopathology
KW - Immunohistochemistry
UR - http://www.scopus.com/inward/record.url?scp=84959107058&partnerID=8YFLogxK
U2 - 10.1016/j.tvjl.2015.10.039
DO - 10.1016/j.tvjl.2015.10.039
M3 - Article
C2 - 26831167
SN - 1090-0233
VL - 209
SP - 125
EP - 132
JO - Veterinary Journal
JF - Veterinary Journal
ER -
