Preparative isolation of the two forms of pig pancreatic pro-(carboxypeptidase A) and their monomeric carboxypeptidases A

M. Vilanova, J. Vendrell, T. Lopez, C. M. Cuchillo, F. X. Avilés

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16 Citations (Scopus)

Abstract

A method is reported for the preparative isolation of the two forms of pro-(carboxy-peptidase A) from pig pancreas: the monomer and the binary complex with pro-(proteinase E). This method, which is mainly based on chromatography on DEAE-Sepharose at pH 5.7, allows these proenzymes to be prepared more quickly and in safer conditions than with other reported methods. Undegraded and homogeneous carboxypeptidase A1 and A2 species (peptidyl-L-amino acid hydrolase, EC 3.4.17.1), in monomeric forms with high specific activity, are also obtained in high yield by controlled trypsin activation of either of the pro-(carboxypeptidases A) followed by chromatography on DEAE-Sepharose at pH 5.8 under dissociating conditions (7M-urea).
Original languageEnglish
Pages (from-to)605-609
JournalBiochemical Journal
Volume229
Issue number3
DOIs
Publication statusPublished - 1 Jan 1985

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