Preparation and characterization of iron-containing liposomes: Their effect on soluble iron uptake by Caco-2 cells

Laura G. Hermida, Anna Roig, Carlos Bregni, Manuel Sabés-Xamaní, Ramon Barnadas-Rodríguez

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Abstract

The aim of this work was to study the iron uptake of Caco-2 cells incubated with five different formulations of liposomes containing iron. The vesicles were also characterized before, during, and after in vitro digestion. Caco-2 cells were incubated with digested and nondigested liposomes, and soluble iron uptake was determined. Nondigested liposomes made with chitosan (CHI) or the cationic lipid, DC-Cholesterol (DC-CHOL), generated the highest iron uptake. However, these two formulations were highly unstable under in vitro digestion, resulting in nonmeasurable iron uptake. Digested conventional liposomes composed of soybean phosphatidylcholine (SPC), hydrogentated phosphatidylcholine (HSPC), or HSPC and cholesterol (CHOL) presented the highest iron-uptake values. These liposomal formulations protected iron from oxidation and improved iron uptake from intestinal cells, compared to an aqueous solution of ferrous sulphate. © 2011 Informa Healthcare USA, Inc.
Original languageEnglish
Pages (from-to)203-212
JournalJournal of Liposome Research
Volume21
Issue number3
DOIs
Publication statusPublished - 1 Sep 2011

Keywords

  • Caco-2 cells
  • Chitosan
  • DC-Cholesterol
  • Phospholipid vesicles

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