TY - JOUR
T1 - Preparation and characterization of iron-containing liposomes: Their effect on soluble iron uptake by Caco-2 cells
AU - Hermida, Laura G.
AU - Roig, Anna
AU - Bregni, Carlos
AU - Sabés-Xamaní, Manuel
AU - Barnadas-Rodríguez, Ramon
PY - 2011/9/1
Y1 - 2011/9/1
N2 - The aim of this work was to study the iron uptake of Caco-2 cells incubated with five different formulations of liposomes containing iron. The vesicles were also characterized before, during, and after in vitro digestion. Caco-2 cells were incubated with digested and nondigested liposomes, and soluble iron uptake was determined. Nondigested liposomes made with chitosan (CHI) or the cationic lipid, DC-Cholesterol (DC-CHOL), generated the highest iron uptake. However, these two formulations were highly unstable under in vitro digestion, resulting in nonmeasurable iron uptake. Digested conventional liposomes composed of soybean phosphatidylcholine (SPC), hydrogentated phosphatidylcholine (HSPC), or HSPC and cholesterol (CHOL) presented the highest iron-uptake values. These liposomal formulations protected iron from oxidation and improved iron uptake from intestinal cells, compared to an aqueous solution of ferrous sulphate. © 2011 Informa Healthcare USA, Inc.
AB - The aim of this work was to study the iron uptake of Caco-2 cells incubated with five different formulations of liposomes containing iron. The vesicles were also characterized before, during, and after in vitro digestion. Caco-2 cells were incubated with digested and nondigested liposomes, and soluble iron uptake was determined. Nondigested liposomes made with chitosan (CHI) or the cationic lipid, DC-Cholesterol (DC-CHOL), generated the highest iron uptake. However, these two formulations were highly unstable under in vitro digestion, resulting in nonmeasurable iron uptake. Digested conventional liposomes composed of soybean phosphatidylcholine (SPC), hydrogentated phosphatidylcholine (HSPC), or HSPC and cholesterol (CHOL) presented the highest iron-uptake values. These liposomal formulations protected iron from oxidation and improved iron uptake from intestinal cells, compared to an aqueous solution of ferrous sulphate. © 2011 Informa Healthcare USA, Inc.
KW - Caco-2 cells
KW - Chitosan
KW - DC-Cholesterol
KW - Phospholipid vesicles
U2 - 10.3109/08982104.2010.517536
DO - 10.3109/08982104.2010.517536
M3 - Article
VL - 21
SP - 203
EP - 212
JO - Journal of Liposome Research
JF - Journal of Liposome Research
SN - 0898-2104
IS - 3
ER -