Prediction and Fourier Transform Infrared Spectroscopy estimation of the secondary structure of a Bacillus licheniformis endo-β-1,3-1,4-d-glucanase

Enrique Querol, Esteve Padrós, Miquel Juncosa, Antoni Planas, Jorge Lloberas

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Abstract

The secondary structure of a recombinant Bacillus licheniformis endo-β-1,3-1,4-d-glucanase (EC.3.2.1.73) has been estimated by Fourier Transform Infrared Spectroscopy and also predicted by the algorithm of Chou and Fasman. From the curve fitting of the deconvolved IR spectrum, the most probable distribution of the secondary structural classes appears to be about 40% β-sheet, 25% reverse turn, 24% non-ordered and 11% α-helix. From theoretical prediction of secondary structure the protein would present 37 % β-sheet, 31% reverse turn, 22% non-ordered and 10% α-helix. © 1992.
Original languageEnglish
Pages (from-to)612-617
JournalBiochemical and Biophysical Research Communications
Volume184
Issue number2
DOIs
Publication statusPublished - 30 Apr 1992

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