Prediction and Fourier Transform Infrared Spectroscopy estimation of the secondary structure of a Bacillus licheniformis endo-β-1,3-1,4-d-glucanase

Enrique Querol; Esteve Padrós; Miquel Juncosa; Antoni Planas; Jorge Lloberas

doi:10.1016/0006-291X(92)90633-V

Prediction and Fourier Transform Infrared Spectroscopy estimation of the secondary structure of a Bacillus licheniformis endo-β-1,3-1,4-d-glucanase

Enrique Querol, Esteve Padrós, Miquel Juncosa, Antoni Planas, Jorge Lloberas

Research output: Contribution to journal › Article › Research › peer-review

3 Citations (Scopus)

Abstract

The secondary structure of a recombinant Bacillus licheniformis endo-β-1,3-1,4-d-glucanase (EC.3.2.1.73) has been estimated by Fourier Transform Infrared Spectroscopy and also predicted by the algorithm of Chou and Fasman. From the curve fitting of the deconvolved IR spectrum, the most probable distribution of the secondary structural classes appears to be about 40% β-sheet, 25% reverse turn, 24% non-ordered and 11% α-helix. From theoretical prediction of secondary structure the protein would present 37 % β-sheet, 31% reverse turn, 22% non-ordered and 10% α-helix. © 1992.

Original language	English
Pages (from-to)	612-617
Journal	Biochemical and Biophysical Research Communications
Volume	184
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(92)90633-V
Publication status	Published - 30 Apr 1992

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title = "Prediction and Fourier Transform Infrared Spectroscopy estimation of the secondary structure of a Bacillus licheniformis endo-β-1,3-1,4-d-glucanase",

abstract = "The secondary structure of a recombinant Bacillus licheniformis endo-β-1,3-1,4-d-glucanase (EC.3.2.1.73) has been estimated by Fourier Transform Infrared Spectroscopy and also predicted by the algorithm of Chou and Fasman. From the curve fitting of the deconvolved IR spectrum, the most probable distribution of the secondary structural classes appears to be about 40% β-sheet, 25% reverse turn, 24% non-ordered and 11% α-helix. From theoretical prediction of secondary structure the protein would present 37 % β-sheet, 31% reverse turn, 22% non-ordered and 10% α-helix. {\textcopyright} 1992.",

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Prediction and Fourier Transform Infrared Spectroscopy estimation of the secondary structure of a Bacillus licheniformis endo-β-1,3-1,4-d-glucanase. / Querol, Enrique; Padrós, Esteve; Juncosa, Miquel; Planas, Antoni; Lloberas, Jorge.

In: Biochemical and Biophysical Research Communications, Vol. 184, No. 2, 30.04.1992, p. 612-617.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Prediction and Fourier Transform Infrared Spectroscopy estimation of the secondary structure of a Bacillus licheniformis endo-β-1,3-1,4-d-glucanase

AU - Querol, Enrique

AU - Padrós, Esteve

AU - Juncosa, Miquel

AU - Planas, Antoni

AU - Lloberas, Jorge

PY - 1992/4/30

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AB - The secondary structure of a recombinant Bacillus licheniformis endo-β-1,3-1,4-d-glucanase (EC.3.2.1.73) has been estimated by Fourier Transform Infrared Spectroscopy and also predicted by the algorithm of Chou and Fasman. From the curve fitting of the deconvolved IR spectrum, the most probable distribution of the secondary structural classes appears to be about 40% β-sheet, 25% reverse turn, 24% non-ordered and 11% α-helix. From theoretical prediction of secondary structure the protein would present 37 % β-sheet, 31% reverse turn, 22% non-ordered and 10% α-helix. © 1992.

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