Prediction and Fourier-transform infrared-spectroscopy estimation of the secondary structure of a recombinant β-glucosidase from Streptomyces sp. (ATCC 11238)

J. A. Perez-Pons, E. Padros, E. Querol

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6 Citations (Scopus)

Abstract

The secondary structure of a recombinant β-glucosidase (EC 3.2.1.21) from Streptomyces sp. (ATCC 11238) has been predicted by computer algorithms and also estimated by Fourier-transform IR spectroscopy. From curve fitting of the deconvoluted IR spectra, the most probable distribution of the secondary-structural classes appears to be about 34% α-helix, 30% β-sheet, 25% reverse turns and 11% non-ordered structures. These data showed a good agreement with data from computer prediction (35% α-helix, 23% β-sheet, 31% reverse turns and 11% non-ordered structures).
Original languageEnglish
Pages (from-to)791-794
JournalBiochemical Journal
Volume308
Issue number3
DOIs
Publication statusPublished - 1 Jan 1995

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