The secondary structure of a recombinant β-glucosidase (EC 126.96.36.199) from Streptomyces sp. (ATCC 11238) has been predicted by computer algorithms and also estimated by Fourier-transform IR spectroscopy. From curve fitting of the deconvoluted IR spectra, the most probable distribution of the secondary-structural classes appears to be about 34% α-helix, 30% β-sheet, 25% reverse turns and 11% non-ordered structures. These data showed a good agreement with data from computer prediction (35% α-helix, 23% β-sheet, 31% reverse turns and 11% non-ordered structures).
|Publication status||Published - 1 Jan 1995|