Prediction and Fourier-transform infrared-spectroscopy estimation of the secondary structure of a recombinant β-glucosidase from Streptomyces sp. (ATCC 11238)

J. A. Perez-Pons; E. Padros; E. Querol

doi:10.1042/bj3080791

Prediction and Fourier-transform infrared-spectroscopy estimation of the secondary structure of a recombinant β-glucosidase from Streptomyces sp. (ATCC 11238)

J. A. Perez-Pons, E. Padros, E. Querol

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Abstract

The secondary structure of a recombinant β-glucosidase (EC 3.2.1.21) from Streptomyces sp. (ATCC 11238) has been predicted by computer algorithms and also estimated by Fourier-transform IR spectroscopy. From curve fitting of the deconvoluted IR spectra, the most probable distribution of the secondary-structural classes appears to be about 34% α-helix, 30% β-sheet, 25% reverse turns and 11% non-ordered structures. These data showed a good agreement with data from computer prediction (35% α-helix, 23% β-sheet, 31% reverse turns and 11% non-ordered structures).

Original language	English
Pages (from-to)	791-794
Journal	Biochemical Journal
Volume	308
Issue number	3
DOIs	https://doi.org/10.1042/bj3080791
Publication status	Published - 1 Jan 1995

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title = "Prediction and Fourier-transform infrared-spectroscopy estimation of the secondary structure of a recombinant β-glucosidase from Streptomyces sp. (ATCC 11238)",

abstract = "The secondary structure of a recombinant β-glucosidase (EC 3.2.1.21) from Streptomyces sp. (ATCC 11238) has been predicted by computer algorithms and also estimated by Fourier-transform IR spectroscopy. From curve fitting of the deconvoluted IR spectra, the most probable distribution of the secondary-structural classes appears to be about 34% α-helix, 30% β-sheet, 25% reverse turns and 11% non-ordered structures. These data showed a good agreement with data from computer prediction (35% α-helix, 23% β-sheet, 31% reverse turns and 11% non-ordered structures).",

author = "Perez-Pons, {J. A.} and E. Padros and E. Querol",

year = "1995",

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T1 - Prediction and Fourier-transform infrared-spectroscopy estimation of the secondary structure of a recombinant β-glucosidase from Streptomyces sp. (ATCC 11238)

AU - Perez-Pons, J. A.

AU - Padros, E.

AU - Querol, E.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - The secondary structure of a recombinant β-glucosidase (EC 3.2.1.21) from Streptomyces sp. (ATCC 11238) has been predicted by computer algorithms and also estimated by Fourier-transform IR spectroscopy. From curve fitting of the deconvoluted IR spectra, the most probable distribution of the secondary-structural classes appears to be about 34% α-helix, 30% β-sheet, 25% reverse turns and 11% non-ordered structures. These data showed a good agreement with data from computer prediction (35% α-helix, 23% β-sheet, 31% reverse turns and 11% non-ordered structures).

AB - The secondary structure of a recombinant β-glucosidase (EC 3.2.1.21) from Streptomyces sp. (ATCC 11238) has been predicted by computer algorithms and also estimated by Fourier-transform IR spectroscopy. From curve fitting of the deconvoluted IR spectra, the most probable distribution of the secondary-structural classes appears to be about 34% α-helix, 30% β-sheet, 25% reverse turns and 11% non-ordered structures. These data showed a good agreement with data from computer prediction (35% α-helix, 23% β-sheet, 31% reverse turns and 11% non-ordered structures).

U2 - 10.1042/bj3080791

DO - 10.1042/bj3080791

M3 - Article

SN - 0264-6021

VL - 308

SP - 791

EP - 794

JO - Biochemical Journal

JF - Biochemical Journal

IS - 3

ER -

Prediction and Fourier-transform infrared-spectroscopy estimation of the secondary structure of a recombinant β-glucosidase from Streptomyces sp. (ATCC 11238)

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