Predicted peptides from non-structural proteins of porcine reproductive and respiratory syndrome virus are able to induce IFN-γ and IL-10

Alexel Burgara-Estrella, Ivan Díaz, Irene M. Rodríguez-Gómez, Sabine E. Essler, Jesús Hernández, Enric Mateu

Research output: Contribution to journalArticleResearchpeer-review

29 Citations (Scopus)

Abstract

This work describes peptides from non-structural proteins (nsp) of porcine reproductive and respiratory syndrome virus (PRRSV) predicted as potential T cell epitopes by bioinfornatics and tested for their ability to induce IFN-γ and IL-10 responses. Pigs immunized with either genotype 1 or genotype 2 PRRSV attenuated vaccines (n=5/group) and unvaccinated pigs (n = 4) were used to test the peptides. Swine leukocyte antigen haplotype of each pig was also determined. Pigs were initially screened for IFN-γ responses (ELISPOT) and three peptides were identified; two of them in non-conserved segments of nsp2 and nsp5 and the other in a conserved region of nsp5 peptide. Then, peptides were screened for IL-10 inducing properties. Six peptides were found to induce IL-10 release in PBMC and some of them were also able to inhibit IFN-γ responses on PHA-stimulated cells. Interestingly, the IFN-γ low responder pigs against PRRSV were mostly homozygous for their SLA haplotypes. In conclusion, these results indicate that nsp of PRRSV contain T-cell epitopes inducing IFN-γ responses as well as IL-10 inducing segments with inhibitory capabilities. © 2013 by the authors; licensee MDPI, Basel, Switzerland.
Original languageEnglish
Pages (from-to)663-677
JournalViruses
Volume5
Issue number2
DOIs
Publication statusPublished - 11 Feb 2013

Keywords

  • Epitopes
  • IL-10
  • Interferon gamma
  • Non-structural proteins
  • PRRSV

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