Potentiometric biosensor for lysine analysis based on a chemically immobilized lysine oxidase membrane

Javier Saurina; Santiago Hernández-Cassou; Esteve Fàbregas; Salvador Alegret

doi:10.1016/S0003-2670(98)00310-9

Potentiometric biosensor for lysine analysis based on a chemically immobilized lysine oxidase membrane

Javier Saurina, Santiago Hernández-Cassou, Esteve Fàbregas, Salvador Alegret

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

28 Citations (Scopus)

Abstract

An enzymatic lysine electrode is developed for the potentiometric analysis of lysine (Lys). This lysine biosensor consists of a chemically immobilized lysine oxidase membrane attached to an all-solid-state ammonium electrode. In this system, the enzymatic degradation of lysine produces ammonia, which is then detected with the ammonium electrode. The influence of immobilization conditions on the potentiometric response and the lifetime of the electrode was studied. Under optimal conditions, the relationship between the potentiometric response and the log[Lys] was linear between 3x10-5 and 10-3M, the sensitivity was 50mV/decade, the repeatability 1.4%, and the response time t(95%) was 15s. Copyright (C) 1998 Elsevier Science B.V.

Original language	English
Pages (from-to)	49-56
Journal	Analytica Chimica Acta
Volume	371
Issue number	1
DOIs	https://doi.org/10.1016/S0003-2670(98)00310-9
Publication status	Published - 21 Sep 1998

Keywords

All-solid-state ammonium electrode
Immobilized lysine oxidase membranes
Lysine analysis
Potentiometric lysine biosensor

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10.1016/S0003-2670(98)00310-9

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title = "Potentiometric biosensor for lysine analysis based on a chemically immobilized lysine oxidase membrane",

abstract = "An enzymatic lysine electrode is developed for the potentiometric analysis of lysine (Lys). This lysine biosensor consists of a chemically immobilized lysine oxidase membrane attached to an all-solid-state ammonium electrode. In this system, the enzymatic degradation of lysine produces ammonia, which is then detected with the ammonium electrode. The influence of immobilization conditions on the potentiometric response and the lifetime of the electrode was studied. Under optimal conditions, the relationship between the potentiometric response and the log[Lys] was linear between 3x10-5 and 10-3M, the sensitivity was 50mV/decade, the repeatability 1.4%, and the response time t(95%) was 15s. Copyright (C) 1998 Elsevier Science B.V.",

keywords = "All-solid-state ammonium electrode, Immobilized lysine oxidase membranes, Lysine analysis, Potentiometric lysine biosensor",

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T1 - Potentiometric biosensor for lysine analysis based on a chemically immobilized lysine oxidase membrane

AU - Saurina, Javier

AU - Hernández-Cassou, Santiago

AU - Fàbregas, Esteve

AU - Alegret, Salvador

PY - 1998/9/21

Y1 - 1998/9/21

N2 - An enzymatic lysine electrode is developed for the potentiometric analysis of lysine (Lys). This lysine biosensor consists of a chemically immobilized lysine oxidase membrane attached to an all-solid-state ammonium electrode. In this system, the enzymatic degradation of lysine produces ammonia, which is then detected with the ammonium electrode. The influence of immobilization conditions on the potentiometric response and the lifetime of the electrode was studied. Under optimal conditions, the relationship between the potentiometric response and the log[Lys] was linear between 3x10-5 and 10-3M, the sensitivity was 50mV/decade, the repeatability 1.4%, and the response time t(95%) was 15s. Copyright (C) 1998 Elsevier Science B.V.

AB - An enzymatic lysine electrode is developed for the potentiometric analysis of lysine (Lys). This lysine biosensor consists of a chemically immobilized lysine oxidase membrane attached to an all-solid-state ammonium electrode. In this system, the enzymatic degradation of lysine produces ammonia, which is then detected with the ammonium electrode. The influence of immobilization conditions on the potentiometric response and the lifetime of the electrode was studied. Under optimal conditions, the relationship between the potentiometric response and the log[Lys] was linear between 3x10-5 and 10-3M, the sensitivity was 50mV/decade, the repeatability 1.4%, and the response time t(95%) was 15s. Copyright (C) 1998 Elsevier Science B.V.

KW - All-solid-state ammonium electrode

KW - Immobilized lysine oxidase membranes

KW - Lysine analysis

KW - Potentiometric lysine biosensor

U2 - 10.1016/S0003-2670(98)00310-9

DO - 10.1016/S0003-2670(98)00310-9

M3 - Article

VL - 371

SP - 49

EP - 56

IS - 1

ER -

Potentiometric biosensor for lysine analysis based on a chemically immobilized lysine oxidase membrane

Abstract

Keywords

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