TY - JOUR
T1 - Potassium starvation in yeast: Mechanisms of homeostasis revealed by mathematical modeling
AU - Kahm, Matthias
AU - Navarrete, Clara
AU - Llopis-Torregrosa, Vicent
AU - Herrera, Rito
AU - Barreto, Lina
AU - Yenush, Lynne
AU - Ariño, Joaquin
AU - Ramos, Jose
AU - Kschischo, Maik
PY - 2012/6/1
Y1 - 2012/6/1
N2 - The intrinsic ability of cells to adapt to a wide range of environmental conditions is a fundamental process required for survival. Potassium is the most abundant cation in living cells and is required for essential cellular processes, including the regulation of cell volume, pH and protein synthesis. Yeast cells can grow from low micromolar to molar potassium concentrations and utilize sophisticated control mechanisms to keep the internal potassium concentration in a viable range. We developed a mathematical model for Saccharomyces cerevisiae to explore the complex interplay between biophysical forces and molecular regulation facilitating potassium homeostasis. By using a novel inference method ("the reverse tracking algorithm") we predicted and then verified experimentally that the main regulators under conditions of potassium starvation are proton fluxes responding to changes of potassium concentrations. In contrast to the prevailing view, we show that regulation of the main potassium transport systems (Trk1,2 and Nha1) in the plasma membrane is not sufficient to achieve homeostasis. © 2012 Kahm et al.
AB - The intrinsic ability of cells to adapt to a wide range of environmental conditions is a fundamental process required for survival. Potassium is the most abundant cation in living cells and is required for essential cellular processes, including the regulation of cell volume, pH and protein synthesis. Yeast cells can grow from low micromolar to molar potassium concentrations and utilize sophisticated control mechanisms to keep the internal potassium concentration in a viable range. We developed a mathematical model for Saccharomyces cerevisiae to explore the complex interplay between biophysical forces and molecular regulation facilitating potassium homeostasis. By using a novel inference method ("the reverse tracking algorithm") we predicted and then verified experimentally that the main regulators under conditions of potassium starvation are proton fluxes responding to changes of potassium concentrations. In contrast to the prevailing view, we show that regulation of the main potassium transport systems (Trk1,2 and Nha1) in the plasma membrane is not sufficient to achieve homeostasis. © 2012 Kahm et al.
UR - https://ddd.uab.cat/record/112622
U2 - https://doi.org/10.1371/journal.pcbi.1002548
DO - https://doi.org/10.1371/journal.pcbi.1002548
M3 - Article
VL - 8
JO - PLoS Computational Biology
JF - PLoS Computational Biology
SN - 1553-734X
M1 - e1002548
ER -