In this study we have explored the importance of polyamine uptake in the proliferation and biochemical maturation of cerebellar astrocytes in culture. The uptake of polyamines paralleled astrocyte proliferation measured as [3H]thymidine incorporation into the DNA. Inhibition of polyamine uptake did not alter the developmental profile of thymidine incorporation, perhaps due to a compensatory increase in ornithine decarboxylase activity but was able to reduce glutamine synthetase (GS) activity, an enzymatic marker for astrocyte biochemical maturation, from 9 days in vitro. The present results suggest that polyamine uptake plays an important role in the biochemical maturation of astrocytes in culture. © 2002 Lippincott Williams & Wilkins.
- Cell proliferation
- Glutamine synthetase
Dot, J., Danchev, N., Blanco, I., & Rodríguez-Alvarez, J. (2002). Polyamine uptake is necessary for a normal biochemical maturation of astrocytes in culture. NeuroReport, 13(8), 1083-1087. https://doi.org/10.1097/00001756-200206120-00022