In this study we have explored the importance of polyamine uptake in the proliferation and biochemical maturation of cerebellar astrocytes in culture. The uptake of polyamines paralleled astrocyte proliferation measured as [3H]thymidine incorporation into the DNA. Inhibition of polyamine uptake did not alter the developmental profile of thymidine incorporation, perhaps due to a compensatory increase in ornithine decarboxylase activity but was able to reduce glutamine synthetase (GS) activity, an enzymatic marker for astrocyte biochemical maturation, from 9 days in vitro. The present results suggest that polyamine uptake plays an important role in the biochemical maturation of astrocytes in culture. © 2002 Lippincott Williams & Wilkins.
- Cell proliferation
- Glutamine synthetase