Platelet-activating factor (PAF) induces wheal and flare skin reactions independent of mast cell degranulation

K. Krause, A. Giménez-Arnau, E. Martinez-Escala, M. Farré-Albadalejo, M. Abajian, M. K. Church, M. Maurer

Research output: Contribution to journalArticleResearchpeer-review

20 Citations (Scopus)

Abstract

Background Platelet-activating factor (PAF) causes wheal and flare responses which are abrogated by H1-antihistamines giving rise to the hypothesis that PAF-induced wheal development is secondary to histamine release from dermal mast cells. But is this hypothesis correct? Methods Wheal and flare responses were induced by intradermal injection of PAF, codeine and histamine in 14 healthy volunteers. Dermal histamine and PGD2 contractions were measured using microdialysis. Results PAF, unlike histamine and codeine, did not cause a statistically significant rise in mean histamine levels with ten persons showing negligible histamine release. Codeine caused a significant but variable histamine release, ranging from 29 to 282 ng/ml. Codeine, but not PAF or histamine, caused a small but statistically significant release of PGD2. Conclusion Wheal and flare reactions in human skin induced by PAF are not associated with histamine release and, therefore, appear to be independent of mast cell degranulation. © 2013 John Wiley & Sons A/S.
Original languageEnglish
Pages (from-to)256-258
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume68
Issue number2
DOIs
Publication statusPublished - 1 Feb 2013

Keywords

  • histamine
  • microdialysis
  • platelet-activating factor
  • prostaglandin D 2
  • wheal and flare

Fingerprint Dive into the research topics of 'Platelet-activating factor (PAF) induces wheal and flare skin reactions independent of mast cell degranulation'. Together they form a unique fingerprint.

Cite this