Platelet-activating factor acetylhydrolase is mainly associated with electronegative low-density lipoprotein subfraction

Sonia Benítez, José Luis Sánchez-Quesada, Vicent Ribas, Oscar Jorba, Francisco Blanco-Vaca, Francesc González-Sastre, Jordi Ordóñez-Llanos

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85 Citations (Scopus)

Abstract

Background - Electronegative LDL [LDL(-)], a modified subfraction of LDL present in plasma, induces the release of interleukin-8 and monocyte chemotactic protein-1 from cultured endothelial cells. Methods and Results - We demonstrate that platelet- activating factor acetylhydrolase (PAF-AH) is mainly associated with LDL(-). LDL(-) had 5-fold higher PAF-AH activity than the nonelectronegative LDL subfraction [LDL(+)] in both normolipemic and familial hypercholesterolemic subjects. Western blot analysis after SDS-PAGE confirmed these results, because a single band of 44 kDa corresponding to PAF-AH appeared in LDL(-) but not in LDL(+). Nondenaturing polyacrylamide gradient gel electrophoresis demonstrated that PAF-AH was bound to LDL(-) regardless of LDL size. In accordance with the above findings, nonesterified fatty acids, a cleavage product of PAF-AH, were increased in LDL(-) compared with LDL(+). Conclusions - The high PAF-AH activity observed in LDL(-) could be related to the proinflammatory activity of these lipoproteins toward cultured endothelial cells.
Original languageEnglish
Pages (from-to)92-96
JournalCirculation
Volume108
Issue number1
DOIs
Publication statusPublished - 8 Jul 2003

Keywords

  • Fatty acids
  • Inflammation
  • Lipoproteins

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