Plasmid maintenance in Escherichia coli recombinant cultures is dramatically, steadily, and specifically influenced by features of the encoded proteins

Research output: Contribution to journalArticleResearchpeer-review

73 Citations (Scopus)

Abstract

A set of eight closely related plasmid constructs carrying CI857- controlled recombinant genes has been used as a model to study plasmid stability in Escherichia coil, in the absence of antibiotic selection. Plasmid loss rates and relative interdivision times of plasmid-bearing cells and plasmid-free cells have been analyzed throughout prolonged cultures. Whereas the calculated plasmid loss rates are not consistent for a given plasmid and set of conditions, the relative growth fitness of plasmid- bearing cells is highly reproducible. In the absence of gene expression, plasmid maintenance is influenced by the length of the cloned segment, the growth temperature, and the plasmid copy number, but not by the plasmid size. At high, inducing temperatures, the effects of the metabolic burden are eclipsed by the toxicity exhibited by the different proteins produced, which is determined by structural features. Despite the multifactorial nature of the negative pressures acting independently on plasmid-bearing cells, the relative cell fitness in a mixed cell population is very reproducible for a given vector, resulting in a monotonous spread of the plasmid-free cells in recombinant cultures.
Original languageEnglish
Pages (from-to)625-632
JournalBiotechnology and Bioengineering
Volume58
Issue number6
DOIs
Publication statusPublished - 20 Jun 1998

Keywords

  • E. coli
  • Partitioning
  • Plasmid
  • Recombinant proteins
  • Selective pressures
  • Toxicity

Fingerprint Dive into the research topics of 'Plasmid maintenance in Escherichia coli recombinant cultures is dramatically, steadily, and specifically influenced by features of the encoded proteins'. Together they form a unique fingerprint.

  • Cite this