Plasmid maintenance and recombinant cell fitness explored in bacterial colonies

Daniel Cazorla, Neus Ferrer-Miralles, Jordi X. Feliu, Xavier Carbonell, Antonio Villaverde

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

During growth of recombinant bacteria, irregular plasmid partitioning generates non-productive, plasmid-free cells whose proportion usually increases in the culture. For Escherichia coli producing engineered β-galactosidases, we have shown a coincidence between plasmid stability and the extension of white/blue areas within individual colonies on X-gal plates. In this context, a good correlation between plasmid permanence in colonies and parameters accurately describing the dynamics of plasmid-free cell population in liquid cultures has been observed. Moreover, the impact of lacZ gene engineering and the metabolic burden imposed by the encoded proteins has been evaluated through plasmid stability by simple image analysis, revealing an enhanced plasmid loss rate as the cells enter into the stationary phase that is modulated by the expression of particular recombinant genes.
Original languageEnglish
Pages (from-to)831-838
JournalBiotechnology Letters
Volume23
DOIs
Publication statusPublished - 7 Jul 2001

Keywords

  • Bacterial colonies
  • Escherichia coli
  • Plasmid stability
  • Protein engineering
  • Stationary phase

Fingerprint

Dive into the research topics of 'Plasmid maintenance and recombinant cell fitness explored in bacterial colonies'. Together they form a unique fingerprint.

Cite this