Pioglitazone does not increase cerebral glucose utilisation in a murine model of Alzheimer's disease and decreases it in wild-type mice

E. Galea, D. L. Feinstein, P. Lacombe

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Abstract

Aims/hypothesis: Clinical trials are in progress to test thiazolidinediones in neurodegenerative diseases such as Alzheimer's disease that involve deficiencies in brain glucose metabolism. While thiazolidinediones enhance glucose uptake in non-cerebral tissues, their impact on brain energy metabolism has not been investigated in vivo. We thus determined whether the thiazolidinedione pioglitazone reverses the decrease in cerebral glucose utilisation (CGU) in a model of brain metabolic deficiency related to Alzheimer's disease. Results are relevant to diabetes because millions of diabetic patients take pioglitazone as an insulin-sensitising drug, and diabetes increases the risk of developing Alzheimer's disease. Materials and methods: The regional pattern of CGU was measured with the 2-deoxy [14C] glucose autoradiographic technique in adult awake mice overexpressing transforming growth factor β1 (TGFβ1), and in wild-type littermates. Mice were treated with pioglitazone for 2 months. Results: Measurement of CGU in 27 brain regions confirmed that TGFβ1 overexpression induced hypometabolism across the brain. Pioglitazone did not reverse the effect of TGFβ1 overexpression and decreased regional CGU in control animals by up to 23%. The extent of the regional CGU decrease induced by pioglitazone, but not that induced by TGFβ1, correlated strongly with basal CGU, suggesting that the higher the local metabolic rate the greater the reduction of CGU effected by pioglitazone. Conclusions/interpretation: In contrast to its stimulatory effect in non-cerebral tissues, chronic treatment with pioglitazone decreases CGU in vivo. This evidence does not support the hypothesis that pioglitazone could act as a metabolic enhancer in Alzheimer's disease, and raises the question of how thiazolidinediones could be beneficial in neurodegenerative diseases. © Springer-Verlag 2006.
Original languageEnglish
Pages (from-to)2153-2161
JournalDiabetologia
Volume49
DOIs
Publication statusPublished - 1 Sep 2006

Keywords

  • Alzheimer's disease
  • Cerebral glucose utilisation
  • Murine model
  • Neurodegenerative diseases
  • Peroxisome proliferator-activated receptor gamma
  • Transforming growth factor beta-1

Fingerprint

Dive into the research topics of 'Pioglitazone does not increase cerebral glucose utilisation in a murine model of Alzheimer's disease and decreases it in wild-type mice'. Together they form a unique fingerprint.

Cite this