Photoswitchable dynasore analogs to control endocytosis with light

Núria Camarero; Ana Trapero; Ariadna Pérez-Jiménez; Eric Macia; Alexandre Gomila-Juaneda; Andrés Martín-Quirós; Laura Nevola; Artur Llobet; Amadeu Llebaria; Jordi Hernando; Ernest Giralt; Pau Gorostiza

doi:10.1039/d0sc03820b

Photoswitchable dynasore analogs to control endocytosis with light

Núria Camarero, Ana Trapero, Ariadna Pérez-Jiménez, Eric Macia, Alexandre Gomila-Juaneda, Andrés Martín-Quirós, Laura Nevola, Artur Llobet, Amadeu Llebaria, Jordi Hernando, Ernest Giralt, Pau Gorostiza^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

4 Citations (Scopus)

Abstract

The spatiotemporal control of cellular dynamic processes has great fundamental interest but lacks versatile molecular tools. Dynamin is a key protein in endocytosis and an appealing target to manipulate cell trafficking using patterns of light. We have developed the first photoswitchable small-molecule inhibitors of endocytosis (dynazos), by a stepwise design of the photochromic and pharmacological properties of dynasore, a dynamin inhibitor. We have characterized their photochromism with UV-visible and transient absorption spectroscopy and their biological activity using fluorescence microscopies and flow cytometry. Dynazos are water-soluble, cell permeable, and photostable, and enable fast, single-wavelength photoswitchable inhibition of clathrin-mediated endocytosis at micromolar concentration.

Original language	English
Pages (from-to)	8981-8988
Number of pages	8
Journal	Chemical Science
Volume	11
Issue number	33
DOIs	https://doi.org/10.1039/d0sc03820b
Publication status	Published - 7 Sept 2020

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title = "Photoswitchable dynasore analogs to control endocytosis with light",

abstract = "The spatiotemporal control of cellular dynamic processes has great fundamental interest but lacks versatile molecular tools. Dynamin is a key protein in endocytosis and an appealing target to manipulate cell trafficking using patterns of light. We have developed the first photoswitchable small-molecule inhibitors of endocytosis (dynazos), by a stepwise design of the photochromic and pharmacological properties of dynasore, a dynamin inhibitor. We have characterized their photochromism with UV-visible and transient absorption spectroscopy and their biological activity using fluorescence microscopies and flow cytometry. Dynazos are water-soluble, cell permeable, and photostable, and enable fast, single-wavelength photoswitchable inhibition of clathrin-mediated endocytosis at micromolar concentration.",

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T1 - Photoswitchable dynasore analogs to control endocytosis with light

AU - Camarero, Núria

AU - Trapero, Ana

AU - Pérez-Jiménez, Ariadna

AU - Macia, Eric

AU - Gomila-Juaneda, Alexandre

AU - Martín-Quirós, Andrés

AU - Nevola, Laura

AU - Llobet, Artur

AU - Llebaria, Amadeu

AU - Hernando, Jordi

AU - Giralt, Ernest

AU - Gorostiza, Pau

PY - 2020/9/7

Y1 - 2020/9/7

N2 - The spatiotemporal control of cellular dynamic processes has great fundamental interest but lacks versatile molecular tools. Dynamin is a key protein in endocytosis and an appealing target to manipulate cell trafficking using patterns of light. We have developed the first photoswitchable small-molecule inhibitors of endocytosis (dynazos), by a stepwise design of the photochromic and pharmacological properties of dynasore, a dynamin inhibitor. We have characterized their photochromism with UV-visible and transient absorption spectroscopy and their biological activity using fluorescence microscopies and flow cytometry. Dynazos are water-soluble, cell permeable, and photostable, and enable fast, single-wavelength photoswitchable inhibition of clathrin-mediated endocytosis at micromolar concentration.

AB - The spatiotemporal control of cellular dynamic processes has great fundamental interest but lacks versatile molecular tools. Dynamin is a key protein in endocytosis and an appealing target to manipulate cell trafficking using patterns of light. We have developed the first photoswitchable small-molecule inhibitors of endocytosis (dynazos), by a stepwise design of the photochromic and pharmacological properties of dynasore, a dynamin inhibitor. We have characterized their photochromism with UV-visible and transient absorption spectroscopy and their biological activity using fluorescence microscopies and flow cytometry. Dynazos are water-soluble, cell permeable, and photostable, and enable fast, single-wavelength photoswitchable inhibition of clathrin-mediated endocytosis at micromolar concentration.

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JO - Chemical Science

JF - Chemical Science

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ER -

Photoswitchable dynasore analogs to control endocytosis with light

Abstract

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