Photoelectro-Enzymatic Glucose Reusable Biosensor by Using Dithienylethene Mediators

Augusto Márquez, Sara Santiago, Carlos Domínguez, Xavier Muñoz-Berbel, Gonzalo Guirado*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)


In the development of colorimetric biosensors, the use of electrochromic mediators has been accepted and widely used during decades. The main drawback of these types of enzymatic substrates is the difficult recovery of the initial redox state of the molecule, which can be done electrochemically or by antioxidants addition, complicating the initially simple structure of the biosensor. those strategies are rarely followed Actually, being the disposable biosensor configuration the most extended for this detection mechanisms. Alternatively, we propose the first reported use of a diacid dithienylethene 1,2-bis(5-carboxy-2-methylthien-3-yl)cyclopentene (DTE) photoelectrochromic compound as a substrate of the horseradish peroxidase (HRP). The photoisomerization between the open (DTEo) and closed (DTEc) forms of the molecule and the respective shift in the redox potential allowed the light-induced enzymatic detection of glucose in the glucose oxidase [(GOx)]–HRP cascade system. This fast and easy control over the enzymatic substrate availability by light pulses permits a gradually consumption and the light-regeneration of the biosensor for a number of cycles. We consider the presented results transcendent in the development of reusable and light-controlled photonic biosensing systems.

Original languageEnglish
Pages (from-to)8714-8719
Number of pages6
JournalChemistry - A European Journal
Issue number40
Publication statusPublished - 17 Jul 2020


  • enzymatic mediators
  • glucose sensing
  • optical biosensors
  • photoelectrochromism
  • regenerable biosensors


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