Phosphoglycerate mutase, 2,3 bisphosphoglycerate phosphatase, creatine kinase and enolase activity and isoenzymes in breast carcinoma

N Durany, J Joseph, OM Jimenez, F Climent, PL Fernandez, F Rivera, J Carreras*

*Corresponding author for this work

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61 Citations (Scopus)

Abstract

We have compared the levels of phosphoglycerate mutase (EC 5.4.2.1), 2,3-bisphosphoglycerate phosphatase (EC 3.1.3.13), creatine kinase (EC 2.7.3.2) and enolase (EC 4.2.1.11) activities and the distribution of their isoenzymes in normal breast tissue and in breast carcinoma. Tumour tissue had higher phosphoglycerate mutase and enolase activity than normal tissue. Creatine kinase activity was higher in seven out of 12 tumours. In contrast 2,3-bisphosphoglycerate phosphatase activity was lower. Phosphoglycerate mutase, enolase and 2,3-bisphosphoglycerate phosphatase presented greater changes in the oestrogen receptor-negative/progesterone receptor-negative breast carcinomas than in the steroid receptor-positive tumours. Determined by electrophoresis, type BE phosphoglycerate mutase, type BE creatine kinase and alpha alpha-enolase were the major isoenzymes detected in normal breast tissue. Types alpha gamma and gamma gamma enolase, types MB and MM phosphoglycerate mutase were detected in much lower proportions. In tumours a decrease of phosphoglycerate mutase isoenzymes possessing gamma-type subunit and some increase of enolase isoenzymes possessing gamma-type subunit was observed. No delectable change was observed in the creatine kinase phenotype. (C) 2000 Cancer Research Campaign.

Original languageEnglish
Pages (from-to)20-27
Number of pages8
JournalBr. J. Cancer
Volume82
Issue number1
Publication statusPublished - Jan 2000

Keywords

  • 2,3-bisphosphoglycerate phosphatase
  • creatine kinase
  • enolase
  • phosphoglycerate mutase activity and isoenzymes
  • breast carcinoma
  • NEURON-SPECIFIC ENOLASE
  • ENDOCRINE DIFFERENTIATION
  • C-ERBB-2 PROTEIN
  • HUMAN-BRAIN
  • NEUROENDOCRINE EXPRESSION
  • RECEPTOR STATUS
  • TUMOR CYTOSOLS
  • GAMMA-SUBUNIT
  • BB ISOENZYME
  • CELL-LINES

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