Phenotypic and genotypic characterization of spotted fever group rickettsiae isolated from Catalan Rhipicephalus sanguineus ticks

L. Beati, V. Roux, A. Ortuño, J. Castella, F. Segura Porta, D. Raoult

    Research output: Contribution to journalArticleResearchpeer-review

    58 Citations (Scopus)

    Abstract

    Eighty-nine Rhipicephalus sanguineus ticks and 21 Rhipicephalus bursa ticks collected in Catalonia were tested by the hemolymph test to establish their infection rate with spotted fever group rickettsiae. By Gimenez staining, 11.2% of the R. sanguineus isolates and 0% of the R. bursa isolates were found to contain rickettsia-like organisms. Six spotted fever group rickettsial strains (Bar29, Bar31, Gir4, Tar1, Tar2, and Tar3) were isolated from these ticks and were characterized by phenotypic and genotypic analyses. PCR followed by restriction fragment length polymorphism analysis showed that the six strains were identical and were characterized by the same restriction profiles as a strain, Mtu5, previously isolated from Rhipicephalus turanicus ticks in the South of France. Microimmunofluorescence serotyping, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified organisms, and Western blot (immunoblot) assay with mouse polyclonal sera confirmed this observation. Pulsed field gel electrophoresis of the whole genome of three of the strains showed that, although closely related, the profile of Tar1 was slightly different from that of the Bar strains. Phylogenetic analysis showed that this new rickettsial sero- and genotype, which will be named the 'Catalan strain,' is closely related to Rickettsia massiliae. This strain shows an unexpected resistance to rifampin. The epidemiological implications of these findings are considered.
    Original languageEnglish
    Pages (from-to)2688-2694
    JournalJournal of Clinical Microbiology
    Volume34
    Issue number11
    Publication statusPublished - 1 Nov 1996

    Fingerprint Dive into the research topics of 'Phenotypic and genotypic characterization of spotted fever group rickettsiae isolated from Catalan Rhipicephalus sanguineus ticks'. Together they form a unique fingerprint.

    Cite this