TY - JOUR
T1 - Phagomagnetic immunoassay for the rapid detection of Salmonella
AU - Laube, Tamara
AU - Cortés, Pilar
AU - Llagostera, Montserrat
AU - Alegret, Salvador
AU - Pividori, María Isabel
N1 - Funding Information:
Acknowledgments Financial support from Ministry of Science and Innovation (MEC), Madrid (Project BIO2010-17566) and from Generalitat de Catalunya (Projects SGR323 and SGR1106), are acknowledged. T.L. also acknowledges the PhD grant of the Comissionat per a Universitats i Recerca del DIUE de la Generalitat de Catalunya i del Fons Social Europeu. Finally, we want to thank Joan Colom and Susana Escribano (Microbiology group) for her excellent technical assistance.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/2/1
Y1 - 2014/2/1
N2 - This work explores the use of the phage P22 in a phagomagnetic immunoassay for the rapid detection of Salmonella. The covalent attachment of wild-type phages was performed on two different magnetic carriers: carboxyl-activated magnetic nanoparticles (300 nm) and tosyl-activated magnetic microparticles (2.8 μm). The bacteria were captured and preconcentrated by the phage-modified magnetic particles, followed by the detection using specific anti-Salmonella antibodies conjugated to horseradish peroxidase as an optical reporter. Outstanding selectivity and sensitivity was obtained with this approach, achieving detection limits of 19 CFU mL-1 in 2.5 h without any pre-enrichment, in milk samples. Moreover, if the samples were pre-enriched for 6 h, the method was able to detect as low as 1.4 CFU in 25 mL of milk. Therefore, the proposed strategy based on the combined use of phagomagnetic separation with immunological labeling is promising as a rapid and simple method for food safety. © 2013 Springer-Verlag Berlin Heidelberg.
AB - This work explores the use of the phage P22 in a phagomagnetic immunoassay for the rapid detection of Salmonella. The covalent attachment of wild-type phages was performed on two different magnetic carriers: carboxyl-activated magnetic nanoparticles (300 nm) and tosyl-activated magnetic microparticles (2.8 μm). The bacteria were captured and preconcentrated by the phage-modified magnetic particles, followed by the detection using specific anti-Salmonella antibodies conjugated to horseradish peroxidase as an optical reporter. Outstanding selectivity and sensitivity was obtained with this approach, achieving detection limits of 19 CFU mL-1 in 2.5 h without any pre-enrichment, in milk samples. Moreover, if the samples were pre-enriched for 6 h, the method was able to detect as low as 1.4 CFU in 25 mL of milk. Therefore, the proposed strategy based on the combined use of phagomagnetic separation with immunological labeling is promising as a rapid and simple method for food safety. © 2013 Springer-Verlag Berlin Heidelberg.
KW - Bacteriophage
KW - Food safety
KW - Magnetic particles
KW - P22
KW - Salmonella
UR - http://www.scopus.com/inward/record.url?scp=84894369489&partnerID=8YFLogxK
U2 - https://doi.org/10.1007/s00253-013-5434-4
DO - https://doi.org/10.1007/s00253-013-5434-4
M3 - Article
C2 - 24362855
VL - 98
SP - 1795
EP - 1805
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 4
ER -