Pesticide determination in tap water and juice samples using disposable amperometric biosensors made using thick-film technology

Miquel Albareda-Sirvent; Arben Merkoçi; Salvador Alegret

doi:10.1016/S0003-2670(01)01017-0

Pesticide determination in tap water and juice samples using disposable amperometric biosensors made using thick-film technology

Miquel Albareda-Sirvent, Arben Merkoçi, Salvador Alegret

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

77 Citations (Scopus)

Abstract

A new design of biosensor strips for pesticide analysis is presented. The developed biosensors integrate a photolithographic conducting copper track, graphite-epoxy composite applied by screen-printing and enzyme (AChE or BChE) immobilised manually by crosslinking with glutaraldehyde. Detection limits in the order of 10-10 to 10-11 M pesticides (paraoxon and carbofuran) have been achieved in standard solutions. Pesticide analysis have been realised on spiked real samples of tap water and fruit juice, with recovering percentages close to 100%, corroborating that the biosensor is sensitive enough to detect pesticides in these matrices. © 2001 Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	35-44
Journal	Analytica Chimica Acta
Volume	442
Issue number	1
DOIs	https://doi.org/10.1016/S0003-2670(01)01017-0
Publication status	Published - 31 Aug 2001

Keywords

Amperometric biosensor
Carbamate pesticides
Cholinesterase-based biosensor
Organophosphorous pesticides
Pesticides
Screen-printed biosensor
Thick-film biosensor

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title = "Pesticide determination in tap water and juice samples using disposable amperometric biosensors made using thick-film technology",

abstract = "A new design of biosensor strips for pesticide analysis is presented. The developed biosensors integrate a photolithographic conducting copper track, graphite-epoxy composite applied by screen-printing and enzyme (AChE or BChE) immobilised manually by crosslinking with glutaraldehyde. Detection limits in the order of 10-10 to 10-11 M pesticides (paraoxon and carbofuran) have been achieved in standard solutions. Pesticide analysis have been realised on spiked real samples of tap water and fruit juice, with recovering percentages close to 100%, corroborating that the biosensor is sensitive enough to detect pesticides in these matrices. {\textcopyright} 2001 Elsevier Science B.V. All rights reserved.",

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AB - A new design of biosensor strips for pesticide analysis is presented. The developed biosensors integrate a photolithographic conducting copper track, graphite-epoxy composite applied by screen-printing and enzyme (AChE or BChE) immobilised manually by crosslinking with glutaraldehyde. Detection limits in the order of 10-10 to 10-11 M pesticides (paraoxon and carbofuran) have been achieved in standard solutions. Pesticide analysis have been realised on spiked real samples of tap water and fruit juice, with recovering percentages close to 100%, corroborating that the biosensor is sensitive enough to detect pesticides in these matrices. © 2001 Elsevier Science B.V. All rights reserved.

KW - Amperometric biosensor

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KW - Cholinesterase-based biosensor

KW - Organophosphorous pesticides

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KW - Thick-film biosensor

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