TY - JOUR
T1 - Pesticide determination by enzymatic inhibition and amperometric detection in a low-temperature cofired ceramics microsystem
AU - Llopis, Xavier
AU - Ibáñez-García, Nuria
AU - Alegret, Salvador
AU - Alonso, Julián
PY - 2007/5/15
Y1 - 2007/5/15
N2 - Among the several fabrication techniques used to construct microflow systems, the low-temperature cofired ceramics (LTCC) technology, taking advantage of its multilayer approach, is one of the most versatile ones. It permits the integration of several unitary operations of an analytical process in a modular or monolithic way. Moreover, due to its perfect compatibility with screen-printing techniques, it also permits the integration of electronic components used to control the whole system setup. In this work the design, construction, and evaluation of a miniaturized analyzer for pesticide determination that integrates a pretreatment stage, based on two mixers or reactors, and an amperometric detection system to measure the product of an enzymatic inhibition reaction are presented. The detection system was monolithically integrated in the microfluidic platform, and it consisted of a screen-printed reference electrode and two platinum sheets, acting as auxiliary and working electrodes, which were embedded within the ceramic structure. The miniaturized system was characterized and successfully evaluated by determining carbofuran at the nanomolar level. © 2007 American Chemical Society.
AB - Among the several fabrication techniques used to construct microflow systems, the low-temperature cofired ceramics (LTCC) technology, taking advantage of its multilayer approach, is one of the most versatile ones. It permits the integration of several unitary operations of an analytical process in a modular or monolithic way. Moreover, due to its perfect compatibility with screen-printing techniques, it also permits the integration of electronic components used to control the whole system setup. In this work the design, construction, and evaluation of a miniaturized analyzer for pesticide determination that integrates a pretreatment stage, based on two mixers or reactors, and an amperometric detection system to measure the product of an enzymatic inhibition reaction are presented. The detection system was monolithically integrated in the microfluidic platform, and it consisted of a screen-printed reference electrode and two platinum sheets, acting as auxiliary and working electrodes, which were embedded within the ceramic structure. The miniaturized system was characterized and successfully evaluated by determining carbofuran at the nanomolar level. © 2007 American Chemical Society.
U2 - https://doi.org/10.1021/ac062390z
DO - https://doi.org/10.1021/ac062390z
M3 - Article
VL - 79
SP - 3662
EP - 3666
ER -