TY - JOUR
T1 - Persistent phagocytic characteristics of microglia in the substantia nigra of long-term parkinsonian macaques
AU - Barcia, Carlos
AU - Ros, Carmen María
AU - Ros-Bernal, Francisco
AU - Gómez, Aurora
AU - Annese, Valentina
AU - Carrillo-de Sauvage, María Angeles
AU - Yuste, José Enrique
AU - Campuzano, Carmen María
AU - de Pablos, Vicente
AU - Fernández-Villalba, Emiliano
AU - Herrero, María Trinidad
N1 - Funding Information:
This work was supported by grants from the Spanish Ministry of Science ( SAF2010-21274 , SAF2007-062262 , FIS PI10-02827 and RYC-2010-06729 ), Fundación Séneca ( FS/15329/PI/10 ) and CIBERNED (Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas). We would like to thank all of the personnel from the SAI (Servicio de Apoyo a la Investigación) for the help they provided at the University of Murcia, especially María García. Finally, the authors declare that there is no conflict of interest related to the present work.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013/8/15
Y1 - 2013/8/15
N2 - Patients with Parkinson's disease show persistent microglial activation in the areas of the brain where the degeneration of dopaminergic neurons takes place. The reason for maintaining this activated state is still unknown, but it is thought that this persistent microglial activation may contribute to the degeneration of dopaminergic neurons. In this study, we report the microanatomical details of microglia and the relationship between microglia and neurons in the substantia nigra pars compacta of Parkinsonian monkeys years after insult with MPTP. We observed that microglial cells appear polarized toward dopaminergic neurons in MPTP-treated macaques compared to untreated animals and present clear phagocytic characteristics, such as engulfing gliaptic contacts, an increase in Golgi apparatus protein machinery and ball-and-chain phagocytic buds. These results demonstrate that activated microglia maintain phagocytic characteristics years after neurotoxin insult, and phagocytosis may be a key contributor to the neurodegenerative process.
AB - Patients with Parkinson's disease show persistent microglial activation in the areas of the brain where the degeneration of dopaminergic neurons takes place. The reason for maintaining this activated state is still unknown, but it is thought that this persistent microglial activation may contribute to the degeneration of dopaminergic neurons. In this study, we report the microanatomical details of microglia and the relationship between microglia and neurons in the substantia nigra pars compacta of Parkinsonian monkeys years after insult with MPTP. We observed that microglial cells appear polarized toward dopaminergic neurons in MPTP-treated macaques compared to untreated animals and present clear phagocytic characteristics, such as engulfing gliaptic contacts, an increase in Golgi apparatus protein machinery and ball-and-chain phagocytic buds. These results demonstrate that activated microglia maintain phagocytic characteristics years after neurotoxin insult, and phagocytosis may be a key contributor to the neurodegenerative process.
KW - Cell polarity
KW - DAPI
KW - Dopaminergic degeneration
KW - GM130
KW - Iba-1
KW - Inflammation
KW - Microglial motility
KW - MPTP
KW - Parkinson's disease
KW - PD
KW - SNpc
KW - TH
UR - http://www.scopus.com/inward/record.url?scp=84880846013&partnerID=8YFLogxK
U2 - 10.1016/j.jneuroim.2013.05.001
DO - 10.1016/j.jneuroim.2013.05.001
M3 - Article
C2 - 23759319
AN - SCOPUS:84880846013
SN - 0165-5728
VL - 261
SP - 60
EP - 66
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1-2
ER -