Performance of VITEK-2 Compact and overnight MicroScan panels for direct identification and susceptibility testing of Gram-negative bacilli from positive FAN BacT/ALERT blood culture bottles

M. D. Quesada, M. Giménez, S. Molinos, G. Fernández, M. D. Sánchez, R. Rivelo, A. Ramírez, G. Banqué, V. Ausina

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35 Citations (Scopus)

Abstract

We describe the reliability of the VITEK-2 Compact and overnight MicroScan panels for direct identification and susceptibility testing from the BacT/ALERT blood culture system when using FAN (FA and FN) bottles. A simple procedure, in two centrifugation steps, was designed to remove the charcoal particles present in FA and FN bottles. A total of 113 positive blood cultures showing Gram-negative rods were investigated. . Enterobacteriaceae were isolated in 104 cases, and . Pseudomonas aeruginosa in nine. The MicroScan system correctly identified 106 (93.8%) of the 113 isolates. The seven identificaction errors included . P. aeruginosa (three), . Enterobacter cloacae (one), . Escherichia coli (one), . Klebsiella oxytoca (one), and . Klebsiella pneumoniae (one). The VITEK-2 system correctly identified 109 (96.5%) of the 113 samples obtained directly from the blood culture bottles. The four unidentified isolates were . Enterobacter cloacae (two), . Escherichia coli (one), and . P. aeruginosa (one). MicroScan yielded 4/779 (0.5%) very major errors and 28/2825 (0.9%) minor errors. VITEK-2 yielded 2/550 (0.36%) very major errors, 1/1718 (0.05%) major error, and 32/2373 (1.3%) minor errors. Both systems provided excellent identification (correlation of >90%) and susceptibility (correlation of >98%) results. The average times required to obtain identification and susceptibility results using the direct test applied to the VITEK-2 Compact system were 4.57 ± 1.37 h and 6.52 ± 1.64 h, respectively. The VITEK-2 compact system provided results on the same day that the blood culture was found to be positive. © 2009 The Authors. Journal Compilation © 2009 European Society of Clinical Microbiology and Infectious Diseases.
Original languageEnglish
Pages (from-to)137-140
JournalClinical Microbiology and Infection
Volume16
Issue number2
DOIs
Publication statusPublished - 1 Jan 2010

Keywords

  • BacT/ALERT
  • Blood culture
  • FAN bottles
  • MicroScan
  • Vitek

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