TY - JOUR
T1 - Peptide-Based Nanostructured Materials with Intrinsic Proapoptotic Activities in CXCR4+ Solid Tumors
AU - Serna, Naroa
AU - Céspedes, María Virtudes
AU - Sánchez-García, Laura
AU - Unzueta, Ugutz
AU - Sala, Rita
AU - Sánchez-Chardi, Alejandro
AU - Cortés, Francisco
AU - Ferrer-Miralles, Neus
AU - Mangues, Ramón
AU - Vázquez, Esther
AU - Villaverde, Antonio
PY - 2017/8/25
Y1 - 2017/8/25
N2 - © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Protein materials are gaining interest in nanomedicine because of the unique combination of regulatable function and structure. A main application of protein nanoparticles is as vehicles for cell-targeted drug delivery in the form of nanoconjugates, in which a conventional or innovative drug is associated to a carrier protein. Here, a new nanomedical approach based on self-assembling protein nanoparticles is developed in which a chemically homogeneous protein material acts, simultaneously, as vehicle and drug. For that, three proapoptotic peptidic factors are engineered to self-assemble as protein-only, fully stable nanoparticles that escape renal clearance, for the multivalent display of a CXCR4 ligand and the intracellular delivery into CXCR4+ colorectal cancer models. These materials, produced and purified in a single step from bacterial cells, show an excellent biodistribution upon systemic administration and local antitumoral effects. The design and generation of intrinsically therapeutic protein-based materials offer unexpected opportunities in targeted drug delivery based on fully biocompatible, tailor-made constructs.
AB - © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Protein materials are gaining interest in nanomedicine because of the unique combination of regulatable function and structure. A main application of protein nanoparticles is as vehicles for cell-targeted drug delivery in the form of nanoconjugates, in which a conventional or innovative drug is associated to a carrier protein. Here, a new nanomedical approach based on self-assembling protein nanoparticles is developed in which a chemically homogeneous protein material acts, simultaneously, as vehicle and drug. For that, three proapoptotic peptidic factors are engineered to self-assemble as protein-only, fully stable nanoparticles that escape renal clearance, for the multivalent display of a CXCR4 ligand and the intracellular delivery into CXCR4+ colorectal cancer models. These materials, produced and purified in a single step from bacterial cells, show an excellent biodistribution upon systemic administration and local antitumoral effects. The design and generation of intrinsically therapeutic protein-based materials offer unexpected opportunities in targeted drug delivery based on fully biocompatible, tailor-made constructs.
KW - advanced therapies
KW - nanoparticles
KW - protein materials
KW - self-assembly
KW - therapeutic proteins
U2 - 10.1002/adfm.201700919
DO - 10.1002/adfm.201700919
M3 - Article
VL - 27
JO - Advanced Functional Materials
JF - Advanced Functional Materials
SN - 1616-301X
IS - 32
M1 - 1700919
ER -