PCR-Genosensor Rapid Test for Detecting Salmonella

M. Isabel Pividori, Arben Merkoçi, Jordi Barbé, Salvador Alegret

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31 Citations (Scopus)

Abstract

A PCR-genosensor based assay for detecting the IS200 DNA sequence specific to Salmonella spp is reported. This rapid test is based on PCR, where amplicon detection is achieved electrochemically using GEC (graphite-epoxy composite) genosensor. The amplicon is immobilized onto GEC electrodes by simple dry-adsorption and the detection is carried out using an enzymatic labeling system. Results demonstrate that this new electrochemical genosensor fulfils the requirements desired for these devices: easy preparation - as dry-adsorption of DNA is very simple -, robustness, sensitivity, low cost, simple use and fast response. Additionally, the assay can be produced as a kit format, increasing its commercial potentiality. Also, the electrode material can be implemented for screen-printing procedures for the mass production of genosensors.
Original languageEnglish
Pages (from-to)1815-1823
JournalElectroanalysis
Volume15
Issue number23-24
DOIs
Publication statusPublished - 1 Dec 2003

Keywords

  • Amperometric genosensors
  • DNA
  • DNA biosensor
  • Enzyme labeling
  • Graphite-epoxy composite
  • Hybridization
  • IS200
  • Nylon membrane
  • Salmonella spp

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  • Cite this

    Pividori, M. I., Merkoçi, A., Barbé, J., & Alegret, S. (2003). PCR-Genosensor Rapid Test for Detecting Salmonella. Electroanalysis, 15(23-24), 1815-1823. https://doi.org/10.1002/elan.200302764