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Abstract
The aims of this study were, first, to determine Listeria monocytogenes, Salmonella enterica, ser. Typhimurium, and Staphylococcus aureus biofilm counts, structure, and their composition in macromolecules by direct epifluorescence microscopy (DEM); and second, to evaluate the distribution of the components of the produced biofilms through 3D representations obtained by confocal laser scanning microscopy (CLSM). Results showed that all assessed strains have a high capacity to generate biofilms, with counts greater than 7 log CFU cm⁻2 in all cases. The highest and lowest survival percentages were obtained by S. aureus with 94.13 ± 1.77% and S. Typhimurium with 60.31 ± 4.45%, respectively. Biofilm matrix composition in macromolecules was as follows: (a) L. monocytogenes: proteins 83.35 ± 5.81%, polysaccharides 10.98 ± 5.77%, and extracellular DNA 5.67 ± 2.5%; (b) S. Typhimurium: 83.37 ± 3.35%, 0.68 ± 0.77%, and 15.95 ± 3.39%, respectively; and (c) S. aureus: 82.55 ± 2.39%, 8.48 ± 2.07%, and 8.97 ± 2.27%, respectively. The qualitative analysis of the 3D representations of the biofilms formed by the pathogens modelized showed no homogeneity and/or ordered distribution of their components within the biofilm architecture. These findings could lead to the development of addressed cleaning and disinfection alternatives.
Original language | English |
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Article number | 113211 |
Number of pages | 9 |
Journal | LWT - Food Science and Technology |
Volume | 159 |
DOIs | |
Publication status | Published - 1 Apr 2022 |
Keywords
- Biofilms
- CLSM
- DEM
- Foodborne pathogens
- Surface hygiene
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Dive into the research topics of 'Pathogenic mono-species biofilm formation on stainless steel surfaces: Quantitative, qualitative, and compositional study'. Together they form a unique fingerprint.Projects
- 1 Finished
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Alternativas al uso de desinfectantes en la industria alimentaria dirigidas a reducir la supervivencia de listeria monocytogenes y salmonella enterica sobre las superficies
Rodriguez Jerez, J. J., Codoñer Cortes, F. M., Ramón Vidal, D., Ripollés Àvila, C., Cervantes Huaman, B. R. H., Tina Mazaheri & Hasoët, A. S.
1/01/19 → 30/09/22
Project: Research Projects and Other Grants