Pathogen-associated gene expression profiles in rainbow trout macrophages

Dimitar B. Iliev, Giles W. Goetz, Simon MacKenzie, Josep V. Planas, Frederick W. Goetz

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)


Pathogens can be distinctively recognized by the cells of the immune system through interactions between the Pathogen-Associated Molecular Patterns (PAMPs) that they produce and the innate immune receptors of leukocytes. The present paper reports on the PAMP-modulated expression of a group of genes expressed in trout macrophages. The genes were identified in subtracted libraries from lipopolysaccharide (LPS)-stimulated macrophages and their expression was analyzed using quantitative real time PCR following stimulation of the cells with E. coli LPS, poly (I:C) and zymosan; representing Gram-negative bacteria, viruses and fungi, respectively. Genes (SPINT1L, DDIT4L, STEAP4, and TNFAIP3), the expression of which was induced by LPS and zymosan, were not significantly up-regulated by poly(I:C) and the opposite was found for transcripts (HMGB1 and PSMB9) up-regulated by poly(I:C). Overall, the differences in gene expression were greater at a later stage of macrophage activation (24 h) at a time when stimulation with poly(I:C) resulted in substantially different responses as compared to LPS and, to a lesser extent, zymosan. © 2006 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)416-422
JournalComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Publication statusPublished - 1 Dec 2006


  • Gene expression
  • Lipopolysaccharide
  • Macrophage
  • Pathogen-associated molecular patterns
  • Poly(I:C)
  • Rainbow trout
  • Zymosan


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