Pasteurella multocida exbB, exbD and tonB genes are physically linked but independently transcribed

Montserrat Bosch, Elena Garrido, Montserrat Llagostera, Ana M. Pérez De Rozas, Ignacio Badiola, Jordi Barbé

Research output: Contribution to journalArticleResearchpeer-review

34 Citations (Scopus)

Abstract

The exbB, exbD and tonB genes of the Pasteurella multocida animal pathogen have been cloned by complementation of an Escherichia coli tonB mutant. Despite these three genes being physically linked, RT-PCR analysis, lacZ transcriptional fusions and construction of insertional mutants have demonstrated that they do not constitute an operon, but rather are transcribed independently from each other. Furthermore, expression of these three genes is under iron control as revealed by lacZ fusions and Fur titration assay analysis. Moreover, each of these three genes is necessary for the virulence of P. multocida cells and all of them contribute equally to the infectious process of this microorganism. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Original languageEnglish
Pages (from-to)201-208
JournalFEMS Microbiology Letters
Volume210
Issue number2
DOIs
Publication statusPublished - 7 May 2002

Keywords

  • exbB gene
  • exbD gene
  • Iron regulation
  • Overlapping gene
  • Pasteurella multocida
  • tonB gene

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