Pasteurella multocida exbB, exbD and tonB genes are physically linked but independently transcribed

Montserrat Bosch; Elena Garrido; Montserrat Llagostera; Ana M. Pérez De Rozas; Ignacio Badiola; Jordi Barbé

doi:10.1016/S0378-1097(02)00626-2

Pasteurella multocida exbB, exbD and tonB genes are physically linked but independently transcribed

Montserrat Bosch, Elena Garrido, Montserrat Llagostera, Ana M. Pérez De Rozas, Ignacio Badiola, Jordi Barbé

Department of Genetics and Microbiology

Research output: Contribution to journal › Article › Research › peer-review

37 Citations (Scopus)

Abstract

The exbB, exbD and tonB genes of the Pasteurella multocida animal pathogen have been cloned by complementation of an Escherichia coli tonB mutant. Despite these three genes being physically linked, RT-PCR analysis, lacZ transcriptional fusions and construction of insertional mutants have demonstrated that they do not constitute an operon, but rather are transcribed independently from each other. Furthermore, expression of these three genes is under iron control as revealed by lacZ fusions and Fur titration assay analysis. Moreover, each of these three genes is necessary for the virulence of P. multocida cells and all of them contribute equally to the infectious process of this microorganism. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	201-208
Journal	FEMS Microbiology Letters
Volume	210
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(02)00626-2 https://doi.org/10.1111/j.1574-6968.2002.tb11181.x
Publication status	Published - 7 May 2002

Keywords

exbB gene
exbD gene
Iron regulation
Overlapping gene
Pasteurella multocida
tonB gene

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title = "Pasteurella multocida exbB, exbD and tonB genes are physically linked but independently transcribed",

abstract = "The exbB, exbD and tonB genes of the Pasteurella multocida animal pathogen have been cloned by complementation of an Escherichia coli tonB mutant. Despite these three genes being physically linked, RT-PCR analysis, lacZ transcriptional fusions and construction of insertional mutants have demonstrated that they do not constitute an operon, but rather are transcribed independently from each other. Furthermore, expression of these three genes is under iron control as revealed by lacZ fusions and Fur titration assay analysis. Moreover, each of these three genes is necessary for the virulence of P. multocida cells and all of them contribute equally to the infectious process of this microorganism. {\textcopyright} 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.",

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T1 - Pasteurella multocida exbB, exbD and tonB genes are physically linked but independently transcribed

AU - Bosch, Montserrat

AU - Garrido, Elena

AU - Llagostera, Montserrat

AU - Pérez De Rozas, Ana M.

AU - Badiola, Ignacio

AU - Barbé, Jordi

PY - 2002/5/7

Y1 - 2002/5/7

N2 - The exbB, exbD and tonB genes of the Pasteurella multocida animal pathogen have been cloned by complementation of an Escherichia coli tonB mutant. Despite these three genes being physically linked, RT-PCR analysis, lacZ transcriptional fusions and construction of insertional mutants have demonstrated that they do not constitute an operon, but rather are transcribed independently from each other. Furthermore, expression of these three genes is under iron control as revealed by lacZ fusions and Fur titration assay analysis. Moreover, each of these three genes is necessary for the virulence of P. multocida cells and all of them contribute equally to the infectious process of this microorganism. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

AB - The exbB, exbD and tonB genes of the Pasteurella multocida animal pathogen have been cloned by complementation of an Escherichia coli tonB mutant. Despite these three genes being physically linked, RT-PCR analysis, lacZ transcriptional fusions and construction of insertional mutants have demonstrated that they do not constitute an operon, but rather are transcribed independently from each other. Furthermore, expression of these three genes is under iron control as revealed by lacZ fusions and Fur titration assay analysis. Moreover, each of these three genes is necessary for the virulence of P. multocida cells and all of them contribute equally to the infectious process of this microorganism. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

KW - exbB gene

KW - exbD gene

KW - Iron regulation

KW - Overlapping gene

KW - Pasteurella multocida

KW - tonB gene

U2 - 10.1016/S0378-1097(02)00626-2

DO - 10.1016/S0378-1097(02)00626-2

M3 - Article

VL - 210

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EP - 208

IS - 2

ER -

Pasteurella multocida exbB, exbD and tonB genes are physically linked but independently transcribed

Abstract

Keywords

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