Pasteurella multocida contains multiple immunogenic haemin- and haemoglobin-binding proteins

Montserrat Bosch, M. Elena Garrido, Ana M. Pérez De Rozas, Ignacio Badiola, Jordi Barbé, Montserrat Llagostera

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25 Citations (Scopus)

Abstract

Iron-dependent outer membrane proteins (IROMPs) play an important role in bacterial pathogenesis and present several attributes of potential vaccine candidates. TBLASTN analysis of the Pasteurella multocida Pm70 genome using the same molecules of other bacterial pathogens as a query identified eight putative haemin and haemoglobin receptors for this organism. Quantitative binding assays have demonstrated that the proteins PM0040, PM0236, PM0741, PM1081, PM1428, PM0592 and HgbA bind both haemin and haemoglobin, whereas PM0576 and PM1282 ORFs only bind either haemoglobin or haemin, respectively. Furthermore, Western blot analysis showed that P. multocida-infected mice generate specific antibodies against PM0040, PM0236, PM0741, PM1081, PM1428, PM0592 and HgbA proteins. Nevertheless, inoculation of mice with any single one of these receptors alone did not protect against P. multocida infection. © 2004 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)103-112
JournalVeterinary Microbiology
Volume99
DOIs
Publication statusPublished - 5 Apr 2004

Keywords

  • Haemin- and haemoglobin-binding proteins
  • Pasteurella multocida
  • Vaccine

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