Passive immunization with antisperm monoclonal antibody ZAP-7 increases preimplantation embryo mortality in the mouse

Pilar Fuentes, Paz Martı́nez

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Objective: To evaluate the effect of VIC-1 and ZAP-7 antihuman sperm monoclonal antibodies on in vivo fertility in the mouse. Design: A randomized blinded study using a mouse model. Setting: University-based laboratory. Animals: B6CBAF1 mice (n = 6 per experimental group). Intervention(s): Antisperm antibodies were administered intravaginally to female mice before mating. Control mice received no treatment, saline, or nonspecific antibodies. Number and viability of preimplantation embryos were determined by microscopic observation. Mouse sperm, oocytes, and normal preimplantation embryos were used in indirect immunofluorescence assays with antisperm antibodies. The effect of antibody treatment on sperm motility and vitality was evaluated. Main Outcome Measure(s): Antigen expression, sperm motility and vitality, number and viability of embryos. Result(s): ZAP-7 antibody recognizes a sperm antigen expressed in zygotes and early preimplantation embryos. Passive immunization with ZAP-7 increases embryo mortality significantly (more than 40% above controls). Passive immunization with VIC-1 has no deleterious effect. Conclusion(s): ZAP-7 monoclonal antibody disrupts fertilization and embryogenesis in the mouse. (C) 2000 by American Society for Reproductive Medicine.
Original languageEnglish
Pages (from-to)1126-1131
JournalFertility and Sterility
Volume73
Issue number6
DOIs
Publication statusPublished - 1 Jan 2000

Keywords

  • Antisperm monoclonal antibody
  • FA-2 antigen
  • Fertility inhibition
  • Passive immunization
  • Preimplantation embryo mortality
  • VIC-1
  • ZAP-7

Fingerprint Dive into the research topics of 'Passive immunization with antisperm monoclonal antibody ZAP-7 increases preimplantation embryo mortality in the mouse'. Together they form a unique fingerprint.

Cite this