Partial hepatectomy, partial portal vein stenosis and mesenteric lymphadenectomy increase splanchnic mast cell infiltration in the rat

Luis M. Moquillaza, María Angeles Aller, Maria Paz Nava, Luis Santamaría, Patri Vergara, Jaime Arias

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

It is currently believed that portal hypertension induces an inflammatory response in which mast cells may be involved. The aim of this study was to verify the involvement of the intestinal submucosal and mesenteric lymph node mast cells in the splanchnic inflammatory response related to portal hypertension. Mast cell infiltration in the intestine (duodenum, jejunum, ileum, caecum and distal colon) and in the mesenteric lymph node complex (MLC) was measured using a stereological method in sham-operated rats (SO; n = 12), in two experimental models of portal hypertension, chronic (triple partial portal vein ligation, TPVL; n = 12) and transient (microsurgical partial hepatectomy; n = 12) and in rats in which the MLC was resected (n = 12). The small and large bowel submucosal infiltration increases in MLC-resected rats (p = 0.0001), in TPVL rats (p = 0.0001) and in rats with partial hepatectomy (p = 0.0001). An extensive mast cell infiltration in the MLC (p = 0.0001) was found in TPVL rats and in rats with partial hepatectomy (347.40±45.25 and 351.92±99.28/mm3, respectively) in relation to sham-operated rats (135.27±30.28/mm3). We conclude that mast cells could be involved in the splanchnic alterations developed in the surgical experimental models of portal hypertension studied. © 2009 Elsevier GmbH.
Original languageEnglish
Pages (from-to)372-382
JournalActa Histochemica
Volume112
Issue number4
DOIs
Publication statusPublished - 1 Jul 2010

Keywords

  • Mast cells
  • Microsurgery
  • Partial hepatectomy
  • Portal hypertension
  • Rat

Fingerprint Dive into the research topics of 'Partial hepatectomy, partial portal vein stenosis and mesenteric lymphadenectomy increase splanchnic mast cell infiltration in the rat'. Together they form a unique fingerprint.

Cite this