Parkin and synphilin-1 isoform expression changes in Lewy body diseases

Jordi Humbert, Katrin Beyer, Cristina Carrato, José L. Mate, Isidro Ferrer, Aurelio Ariza

Research output: Contribution to journalArticleResearchpeer-review

26 Citations (Scopus)

Abstract

Alternative splicing gives rise to at least seven parkin and eight synphilin-1 isoforms. Since both parkin and synphilin-1 have been involved in Lewy body (LB) formation, we decided to explore whether their isoforms are differentially expressed in LB diseases. With this aim, we studied relative mRNA expression levels of parkin and synphilin-1 isoforms in the frontal cortices of patients with dementia with LBs, the LB variant of Alzheimer's disease and Parkinson's disease and compared the findings with those obtained from Alzheimer's disease patients and control individuals. Duplex real-time PCR reactions, with beta-actin as internal standard, were carried out in a LightCycler. mRNA expression levels of parkin and synphilin-1 isoforms were seen to be specifically altered in each of the LB diseases studied. These findings suggest that parkin and synphilin-1 isoform expression changes play a significant role in the pathogenesis of LB diseases. © 2007 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)681-687
JournalNeurobiology of Disease
Volume26
Issue number3
DOIs
Publication statusPublished - 1 Jun 2007

Keywords

  • Dementia with Lewy bodies
  • Differential isoform expression
  • Lewy body variant of Alzheimer disease
  • Parkinson disease
  • Transcript variant

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