Parasite specific antibody levels, interferon-γ and TLR2 and TLR4 transcripts in blood from dogs with different clinical stages of leishmaniosis

Sara Montserrat-Sangrà, Laura Ordeix, Pamela Martínez-Orellana, Laia Solano-Gallego

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

© 2018 by the authors. Canine leishmaniosis has a wide range of disease severity from mild (stage I), to severe (stages II-III), or very severe disease (stage IV). The objective of the study was to evaluate and compare serum antibody levels, Leishmania infantum specific IFN- γ production and TLR2 and TLR4 transcripts in non-stimulated blood from dogs with different clinical stages at the time of diagnosis as well as blood parasitemia. Enzyme-Linked ImmunoSorbent Assay (ELISAs) were performed to determine serum antibody levels and IFN- γ production and quantitative polymerase chain reaction (qPCRs) in order to determine blood parasite load and TLR2 and TLR4 transcripts. Older dogs were significantly affected by more severe disease with higher antibody levels and blood parasitemia than dogs with mild disease. IFN- γ production was significantly higher in dogs with stage I disease when compared to dogs with more severe disease. Relative quantification of TLR2 in dogs with mild disease was similar to that of control dogs. On the other hand, TLR2 transcripts were significantly higher in dogs with severe disease as compared with that from control healthy dogs. No differences were found in TLR4 relative quantification between groups. This study demonstrates that dogs with different clinical stages of leishmaniosis present different levels of biological markers indicative of different immune responses.
Original languageEnglish
Article number31
JournalVeterinary Sciences
Volume5
Issue number1
DOIs
Publication statusPublished - 1 Mar 2018

Keywords

  • Blood parasitemia
  • Dog
  • IFN-γ
  • Leishmania infantum
  • Papular dermatitis
  • TLR2
  • TLR4

Fingerprint

Dive into the research topics of 'Parasite specific antibody levels, interferon-γ and TLR2 and TLR4 transcripts in blood from dogs with different clinical stages of leishmaniosis'. Together they form a unique fingerprint.

Cite this