Papain immobilization study in enzymatic synthesis of dipeptide gly-phe

Montserrat Capellas, Pere Serra, M. Dolors Benaiges, Gloria Caminal, Gloria Gonzalez, Josep Lopez-Santin

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Papain catalysed peptide synthesis was followed to determine the optimum conditions for adsorption and covalent binding immobilization methods. The synthesis of the dipeptide Gly-Phe was studied in two different reaction systems: a) For adsorbed papain, in an organic medium containing low water concentration, b) For covalently bound enzyme, in a two-liquid phase system, using trichloroethylene as organic phase. The amount of enzyme offered to the immobilization support and the pH of the immobilization procedure were the main parameters studied. © 1994 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Original languageEnglish
Pages (from-to)273-281
JournalBiocatalysis and Biotransformation
Volume11
DOIs
Publication statusPublished - 1 Jan 1994

Keywords

  • Enzymatic peptide synthesis
  • Organic solvents
  • Papain immobilization

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