Pancreas in recent onset insulin-dependent diabetes mellitus: Changes in HLA, adhesion molecules and autoantigens, restricted T cell receptor Vβ usage, and cytokine profile

Nuria Somoza, Francesca Vargas, Carme Roura-Mir, Marta Vives-Pi, María Teresa Fernández-Figueras, Aurelio Ariza, Ramón Gomis, Rafael Bragado, Mercè Martí, Dolores Jaraquemada, Ricardo Pujol-Borrell

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213 Citations (Scopus)

Abstract

Insulin-dependent diabetes mellitus (IDDM), in which only the pancreatic β cells are destroyed by the autoimmune response, is the paradigm of organ- specific autoimmunity. As a result of a combination of factors, the number of immunohistologic/cellular/molecular studies of pancreas in IDDM is very limited. We report here studies conducted in the pancreata of two IDDM patients: one newly diagnosed (case 1) and one long standing (case 2). In case 1, we demonstrated the presence of morphologically normal viable β cells without evidence of viral infection. In both cases the expression of the autoantigens defined by islet cell Abs and by glutamic acid decarboxylase was markedly reduced in the islet cells whereas expression of hsp60, another putative autoantigen, was normal. Over-expression of HLA class I was detected in 58% of the islets in pancreatic sections and in cultured β cells in case 1 and also in 30% of islets in case 2 but it was not restricted to any insular cell type. In case 1, there was 'inappropriate' HLA class II expression in islets cells but it was a rare finding and not β cell specific. The analysis of the correlation between class II overexpression, residual insulin, and insulitis suggests that the first event is the increase of HLA class I expression. Of adhesion molecules, ICAM-1, VLA, VCAM, and LFA- 3 were normal and only ICAM-1 was moderately overexpressed in and around the islets of case 1 insulitis, as was detected by immunofluorescence which showed that 18% of the islets of case 1 had CD8+ lymphocytes as the predominant population. Reverse transcription-PCR demonstrated moderate Vβ skewing and the profile of cytokines expected In CTLs: IL-2, IL-4, IL-10 and IFN-γ negative, perforin positive. In addition, IFN-α, IFN-β, and IL-6 transcripts were detected in the case 1 pancreas, consistent with the existence of a silent viral infection. Overall, the results indicated that, differently from spontaneous animal models of diabetes, in the pancreas of IDDM patients there are no elements of the inductive phase of the autoimmune response.
Original languageEnglish
Pages (from-to)1360-1377
JournalJournal of Immunology
Volume153
Issue number3
Publication statusPublished - 1 Aug 1994

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