p53 Controls Meiotic Prophase Progression and Crossover Formation

Marina Marcet-Ortega, Andros Maldonado-Linares, Maria López-Panadés, Ignasi Roig*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Meiosis initiates with the formation of double strand breaks (DSBs) throughout the genome. To avoid genomic instability, these DSBs need to be correctly repaired by homologous recombination. Surveillance mechanisms involving the DNA damage response (DDR) pathway ATM-CHK2-p53 can detect the persistence of unrepaired DBSs and activate the recombination-dependent arrest at the pachytene stage. However, a complete understanding of p53 functions under normal physiological conditions remains lacking. Here, we report a detailed analysis of the p53 role during meiotic prophase in mice spermatocytes. We show that the absence of p53 regulates prophase progression by slowing down the pachytene stage when the recombination-dependent arrest occurs. Furthermore, our results show that p53 is necessary for proper crossover (CO) formation and localization. Our study contributes to a deeper understanding of p53 roles during the meiotic prophase.

Original languageEnglish
Article number9818
JournalInternational journal of molecular sciences
Volume23
Issue number17
DOIs
Publication statusPublished - 29 Aug 2022

Keywords

  • DSB repair
  • MLH1
  • crossover
  • meiosis
  • p53
  • synaptonemal complex
  • γH2AX

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