Overproduction and purification of an agarase of bacterial origin

Víctor Parro; Carme Vives; Francesc Godia; Rafael P. Mellado

doi:https://doi.org/10.1016/S0168-1656(97)00128-4

Overproduction and purification of an agarase of bacterial origin

Víctor Parro, Carme Vives, Francesc Godia, Rafael P. Mellado

Research output: Contribution to journal › Article › Research › peer-review

6 Citations (Scopus)

Abstract

The agarase gene from Streptomyces coelicolor has been cloned in the non-producer bacterium Streptomyces lividans under the control of its own set of promoters and under the control of a heterologous promoter that is functional only during exponential growth. The best level of overproduction was obtained when the strain containing the natural gene was cultivated in fed batch with mannitol as carbon source. The protein, with a relative molecular mass of 32 kDa, has been purified following an affinity purification method. Contaminating activities seem to be absent from the purified enzyme preparation that can be used to purify DNA from agarose gels.

Original language	English
Pages (from-to)	59-66
Journal	Journal of Biotechnology
Volume	58
DOIs	https://doi.org/10.1016/S0168-1656(97)00128-4
Publication status	Published - 2 Oct 1997

Keywords

Affinity purification
Agarase
Exponential phase promoter
Fed-batch culture
Overproduction

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title = "Overproduction and purification of an agarase of bacterial origin",

abstract = "The agarase gene from Streptomyces coelicolor has been cloned in the non-producer bacterium Streptomyces lividans under the control of its own set of promoters and under the control of a heterologous promoter that is functional only during exponential growth. The best level of overproduction was obtained when the strain containing the natural gene was cultivated in fed batch with mannitol as carbon source. The protein, with a relative molecular mass of 32 kDa, has been purified following an affinity purification method. Contaminating activities seem to be absent from the purified enzyme preparation that can be used to purify DNA from agarose gels.",

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T1 - Overproduction and purification of an agarase of bacterial origin

AU - Parro, Víctor

AU - Vives, Carme

AU - Godia, Francesc

AU - Mellado, Rafael P.

PY - 1997/10/2

Y1 - 1997/10/2

N2 - The agarase gene from Streptomyces coelicolor has been cloned in the non-producer bacterium Streptomyces lividans under the control of its own set of promoters and under the control of a heterologous promoter that is functional only during exponential growth. The best level of overproduction was obtained when the strain containing the natural gene was cultivated in fed batch with mannitol as carbon source. The protein, with a relative molecular mass of 32 kDa, has been purified following an affinity purification method. Contaminating activities seem to be absent from the purified enzyme preparation that can be used to purify DNA from agarose gels.

AB - The agarase gene from Streptomyces coelicolor has been cloned in the non-producer bacterium Streptomyces lividans under the control of its own set of promoters and under the control of a heterologous promoter that is functional only during exponential growth. The best level of overproduction was obtained when the strain containing the natural gene was cultivated in fed batch with mannitol as carbon source. The protein, with a relative molecular mass of 32 kDa, has been purified following an affinity purification method. Contaminating activities seem to be absent from the purified enzyme preparation that can be used to purify DNA from agarose gels.

KW - Affinity purification

KW - Agarase

KW - Exponential phase promoter

KW - Fed-batch culture

KW - Overproduction

U2 - https://doi.org/10.1016/S0168-1656(97)00128-4

DO - https://doi.org/10.1016/S0168-1656(97)00128-4

M3 - Article

VL - 58

SP - 59

EP - 66

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

ER -