An improved approach to optimize the overall sensitivity and the resolution requirements in the indirect dimension of 13C/15N time-shared (TS) NMR experiments is presented. A different data sampling acquisition procedure is applied for 13C and 15N in the indirect dimension, and a proper data recombination before conventional data processing allows a customized adjustment of spectral widths, number of scans and number of increments individually for 13C and 15N. The major benefit is an important improvement on the detection limits of the TS experiment that overcomes the lower sensitivity of 15N over 13C at natural abundance. We evaluate such enhancements from 2D TS-HMBC experiments recorded on a nitrogen-containing synthetic azole derivative of pharmaceutical interest. Copyright © 2007 John Wiley & Sons, Ltd.
|Journal||Magnetic Resonance in Chemistry|
|Publication status||Published - 1 Apr 2007|