Optimized production of MLM triacylglycerols catalyzed by immobilized heterologous Rhizopus oryzae lipase

P. A. Nunes, P. Pires-Cabral, M. Guillén, F. Valero, S. Ferreira-Dias

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25 Citations (Scopus)


Response surface methodology was used to model and optimize the acidolysis of virgin olive oil with caprylic (C8:0) or capric (C10:0) acids, aimed at the production of low caloric triacylglycerols (TAG) of MLM type, in solvent free media, catalyzed by the heterologous Rhizopus oryzae lipase (r-ROL) immobilized in Eupergit ®C. This lipase was produced in the methylotrophic yeast Pichia pastoris Mut s phenotype (experiments with C10:0) or a Mut? phenotype (experiments with C8:0), under different operational conditions. The r-ROL used in experiments with C10:0 presented a hydrolytic activity about 5 times of that presented by r-ROL used in acidolysis with C8:0. The experiments were carried out following a central composite rotatable design, as a function of the molar ratio (MR) medium chain fatty acid/TAG (1.6-4.4) and temperature (25-55 °C). Convex surfaces described by second order polynomials as a function of MR and temperature were well fitted to fatty acid incorporation values. After 24-h reaction, the predicted maximum incorporation of caprylic (15.5 mol%) or capric (33.3 mol%) acids in olive oil occurs at 37 and 35 °C, respectively, and at C8:0/TAG of 2.8:1 or C10:0/TAG of 3:1. These predicted optima were experimentally validated. Fermentation conditions used in r-ROL production highly affected hydrolytic activity and to a lesser extent interesterification activity. © 2012 AOCS.
Original languageEnglish
Pages (from-to)1287-1295
JournalJAOCS, Journal of the American Oil Chemists' Society
Issue number7
Publication statusPublished - 1 Jul 2012


  • Acidolysis
  • Capric acid
  • Caprylic acid
  • Olive oil
  • Recombinant Rhizopus oryzae lipase
  • Response surface methodology
  • Structured lipids


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