In this work it is shown the optimization of the method to detect tenuazonic acid by means of thin layer chromatography, from Alternaria alternata IMI 354942 cultures. The strain was developed in glucosed malt extract at 2%, with an incubation period of seven days at 25 °C. For the extraction and optimization of the analysis of tenuazonic acid, a modification of the technique described by E.E. Stinson et al. (1980) was applied. Cromatography plates were developed in different eluents with the objective to optimize the separation and detection of micotoxin. The identification of tenuazonic acid, produced by the studied strain, was carried out by means of a reference gage. This was detected as a dark spot when the plates were observed under ultraviolet light at 254 nm. The elected solvents system was formed by toluene/methanol/acetic acid int he proportion of 86:12:2.
|Publication status||Published - 1 May 2000|
- Alternaria alternata
- Tenuazonic acid
- Thin layer chromatography