Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review

Oriol Cos, Ramón Ramón, José Luis Montesinos, Francisco Valero

    Research output: Contribution to journalReview articleResearchpeer-review

    241 Citations (Scopus)

    Abstract

    The methylotrophic yeast Pichia pastoris has been widely reported as a suitable expression system for heterologous protein production. The use of different phenotypes under PAOX promoter, other alternative promoters, culture medium, and operational strategies with the objective to maximize either yield or productivity of the heterologous protein, but also to obtain a repetitive product batch to batch to get a robust process for the final industrial application have been reported. Medium composition, kinetics growth, fermentation operational strategies from fed-batch to continuous cultures using different phenotypes with the most common PAOX promoter and other novel promoters (GAP, FLD, ICL), the use of mixed substrates, on-line monitoring of the key fermentation parameters (methanol) and control algorithms applied to the bioprocess are reviewed and discussed in detail. © 2006 Cos et al; licensee BioMed Central Ltd.
    Original languageEnglish
    Number of pages20
    JournalMicrobial Cell Factories
    Volume5
    Issue number17
    DOIs
    Publication statusPublished - 6 Apr 2006

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