Ontogeny of the Major Xenobiotic-Metabolizing Enzymes Expression and the Dietary Lipids Modulatory Effect in the Rat Dimethylbenz(a)anthracene-Induced Breast Cancer Model

Miguel Ángel Manzanares; Montserrat Solanas; Raquel Moral; Raquel Escrich; Elena Vela; Eduard Escrich

doi:10.1002/jbt.21596

Ontogeny of the Major Xenobiotic-Metabolizing Enzymes Expression and the Dietary Lipids Modulatory Effect in the Rat Dimethylbenz(a)anthracene-Induced Breast Cancer Model

Miguel Ángel Manzanares, Montserrat Solanas, Raquel Moral, Raquel Escrich, Elena Vela, Eduard Escrich

Research output: Contribution to journal › Article › Research › peer-review

4 Citations (Scopus)

Abstract

© 2014 Wiley Periodicals, Inc. Breast cancer is the most common malignancy in women worldwide. Environmental factors such as xenobiotic exposure and lifestyle and nutrition play a key role in its etiology. This study was designed to evaluate the age-related changes in the expression of major xenobiotic-metabolizing enzymes (XMEs) in the rat liver and the mammary gland in the dimethylbenz(a)anthracene-induced breast cancer model. The influence of dietary lipids on the ontogeny of XMEs was also evaluated. mRNA and protein levels of phase I (CYP1A1, CYP1A2, and CYP1B1) and phase II (NAD(P)H:quinone acceptor oxidoreductase 1 and GSTP1) enzymes were analyzed, as well as their regulation by AhR and Nrf2, respectively. Results showed differences in the phase I enzymes expression, whereas little changes were obtained in phase II. High corn oil and olive oil diets differentially influenced the expression of age-related changes, suggesting that the different susceptibility to xenobiotic exposure depending upon the age may be modulated by dietary factors.

Original language	English
Pages (from-to)	539-548
Journal	Journal of Biochemical and Molecular Toxicology
Volume	28
DOIs	https://doi.org/10.1002/jbt.21596
Publication status	Published - 1 Dec 2014

Keywords

Breast Cancer Model
Dietary Lipids
Ontogeny
Polycyclic Aromatic Hydrocarbons (PAHs)
Xenobiotic-Metabolizing Enzymes (XMEs)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/jbt.21596

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title = "Ontogeny of the Major Xenobiotic-Metabolizing Enzymes Expression and the Dietary Lipids Modulatory Effect in the Rat Dimethylbenz(a)anthracene-Induced Breast Cancer Model",

abstract = "{\textcopyright} 2014 Wiley Periodicals, Inc. Breast cancer is the most common malignancy in women worldwide. Environmental factors such as xenobiotic exposure and lifestyle and nutrition play a key role in its etiology. This study was designed to evaluate the age-related changes in the expression of major xenobiotic-metabolizing enzymes (XMEs) in the rat liver and the mammary gland in the dimethylbenz(a)anthracene-induced breast cancer model. The influence of dietary lipids on the ontogeny of XMEs was also evaluated. mRNA and protein levels of phase I (CYP1A1, CYP1A2, and CYP1B1) and phase II (NAD(P)H:quinone acceptor oxidoreductase 1 and GSTP1) enzymes were analyzed, as well as their regulation by AhR and Nrf2, respectively. Results showed differences in the phase I enzymes expression, whereas little changes were obtained in phase II. High corn oil and olive oil diets differentially influenced the expression of age-related changes, suggesting that the different susceptibility to xenobiotic exposure depending upon the age may be modulated by dietary factors.",

keywords = "Breast Cancer Model, Dietary Lipids, Ontogeny, Polycyclic Aromatic Hydrocarbons (PAHs), Xenobiotic-Metabolizing Enzymes (XMEs)",

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language = "English",

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journal = "Journal of Biochemical and Molecular Toxicology",

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Ontogeny of the Major Xenobiotic-Metabolizing Enzymes Expression and the Dietary Lipids Modulatory Effect in the Rat Dimethylbenz(a)anthracene-Induced Breast Cancer Model. / Manzanares, Miguel Ángel; Solanas, Montserrat ; Moral, Raquel et al.
In: Journal of Biochemical and Molecular Toxicology, Vol. 28, 01.12.2014, p. 539-548.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Ontogeny of the Major Xenobiotic-Metabolizing Enzymes Expression and the Dietary Lipids Modulatory Effect in the Rat Dimethylbenz(a)anthracene-Induced Breast Cancer Model

AU - Manzanares, Miguel Ángel

AU - Solanas, Montserrat

AU - Moral, Raquel

AU - Escrich, Raquel

AU - Vela, Elena

AU - Escrich, Eduard

PY - 2014/12/1

Y1 - 2014/12/1

N2 - © 2014 Wiley Periodicals, Inc. Breast cancer is the most common malignancy in women worldwide. Environmental factors such as xenobiotic exposure and lifestyle and nutrition play a key role in its etiology. This study was designed to evaluate the age-related changes in the expression of major xenobiotic-metabolizing enzymes (XMEs) in the rat liver and the mammary gland in the dimethylbenz(a)anthracene-induced breast cancer model. The influence of dietary lipids on the ontogeny of XMEs was also evaluated. mRNA and protein levels of phase I (CYP1A1, CYP1A2, and CYP1B1) and phase II (NAD(P)H:quinone acceptor oxidoreductase 1 and GSTP1) enzymes were analyzed, as well as their regulation by AhR and Nrf2, respectively. Results showed differences in the phase I enzymes expression, whereas little changes were obtained in phase II. High corn oil and olive oil diets differentially influenced the expression of age-related changes, suggesting that the different susceptibility to xenobiotic exposure depending upon the age may be modulated by dietary factors.

AB - © 2014 Wiley Periodicals, Inc. Breast cancer is the most common malignancy in women worldwide. Environmental factors such as xenobiotic exposure and lifestyle and nutrition play a key role in its etiology. This study was designed to evaluate the age-related changes in the expression of major xenobiotic-metabolizing enzymes (XMEs) in the rat liver and the mammary gland in the dimethylbenz(a)anthracene-induced breast cancer model. The influence of dietary lipids on the ontogeny of XMEs was also evaluated. mRNA and protein levels of phase I (CYP1A1, CYP1A2, and CYP1B1) and phase II (NAD(P)H:quinone acceptor oxidoreductase 1 and GSTP1) enzymes were analyzed, as well as their regulation by AhR and Nrf2, respectively. Results showed differences in the phase I enzymes expression, whereas little changes were obtained in phase II. High corn oil and olive oil diets differentially influenced the expression of age-related changes, suggesting that the different susceptibility to xenobiotic exposure depending upon the age may be modulated by dietary factors.

KW - Breast Cancer Model

KW - Dietary Lipids

KW - Ontogeny

KW - Polycyclic Aromatic Hydrocarbons (PAHs)

KW - Xenobiotic-Metabolizing Enzymes (XMEs)

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DO - 10.1002/jbt.21596

M3 - Article

SN - 1095-6670

VL - 28

SP - 539

EP - 548

JO - Journal of Biochemical and Molecular Toxicology

JF - Journal of Biochemical and Molecular Toxicology

ER -

Ontogeny of the Major Xenobiotic-Metabolizing Enzymes Expression and the Dietary Lipids Modulatory Effect in the Rat Dimethylbenz(a)anthracene-Induced Breast Cancer Model

Abstract

Keywords

UN SDGs

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