Ontogeny of the Major Xenobiotic-Metabolizing Enzymes Expression and the Dietary Lipids Modulatory Effect in the Rat Dimethylbenz(a)anthracene-Induced Breast Cancer Model

Miguel Ángel Manzanares, Montserrat Solanas, Raquel Moral, Raquel Escrich, Elena Vela, Eduard Escrich

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Abstract

© 2014 Wiley Periodicals, Inc. Breast cancer is the most common malignancy in women worldwide. Environmental factors such as xenobiotic exposure and lifestyle and nutrition play a key role in its etiology. This study was designed to evaluate the age-related changes in the expression of major xenobiotic-metabolizing enzymes (XMEs) in the rat liver and the mammary gland in the dimethylbenz(a)anthracene-induced breast cancer model. The influence of dietary lipids on the ontogeny of XMEs was also evaluated. mRNA and protein levels of phase I (CYP1A1, CYP1A2, and CYP1B1) and phase II (NAD(P)H:quinone acceptor oxidoreductase 1 and GSTP1) enzymes were analyzed, as well as their regulation by AhR and Nrf2, respectively. Results showed differences in the phase I enzymes expression, whereas little changes were obtained in phase II. High corn oil and olive oil diets differentially influenced the expression of age-related changes, suggesting that the different susceptibility to xenobiotic exposure depending upon the age may be modulated by dietary factors.
Original languageEnglish
Pages (from-to)539-548
JournalJournal of Biochemical and Molecular Toxicology
Volume28
DOIs
Publication statusPublished - 1 Dec 2014

Keywords

  • Breast Cancer Model
  • Dietary Lipids
  • Ontogeny
  • Polycyclic Aromatic Hydrocarbons (PAHs)
  • Xenobiotic-Metabolizing Enzymes (XMEs)

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