Online SERS Quantification of Staphylococcus aureus and the Application to Diagnostics in Human Fluids

Carme Catala, Bernat Mir-Simon, Xiaotong Feng, Celia Cardozo, Nicolas Pazos-Perez, Elena Pazos, Sara Gómez-de Pedro, Luca Guerrini, Alex Soriano, Jordi Vila, Francec Marco, Eduardo Garcia-Rico, Ramon A. Alvarez-Puebla

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    46 Citations (Scopus)


    © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Staphylococcus aureus is a common cause of serious infections. One of the main drawbacks in its treatment is the time required for a positive diagnosis, over 24 h, as most methods are still based in bacterial culture. Herein, a microfluidic optical device for the rapid and ultrasensitive quantification of S. aureus in real human fluids is designed. In this approach, the surface-enhanced Raman scattering (SERS)-encoded particles, functionalized with either an antibody or an aptamer, form a dense collection of electromagnetic hot spots on the surface of S. aureus. This allows for an exponentially increase of the SERS signal when particles accumulate on the microorganism as compared to their free condition in bulk solution. Quantification is achieved by passing the sample through a microfluidic device with a collection window where a laser interrogates and classifies each of the induced bacteria–nanoparticle aggregates in real time. Further, the advantages of using aptamers versus antibodies as biorecognition elements are extensively investigated.
    Original languageEnglish
    Article number1600163
    JournalAdvanced Materials Technologies
    Issue number8
    Publication statusPublished - 1 Jan 2016


    • clinical diagnosis
    • infectious diseases
    • S. aureus
    • surface-enhanced Raman scattering (SERS)


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