Abstract
An automated sequential injection analysis (SIA) system using stop-flow technique was developed to determine methanol concentration by means of the enzymatic reactions of alcohol oxidase and peroxidase. Its application as an on-line device for monitoring Pichia pastoris fermentations producing an heterologous protein was demonstrated. Linear response, observed up to 2 g l-1, was reached by including a dilution chamber in the SIA manifold. The sampling frequency was 7 analyses per hour with a relative standard deviation lower than 4%.
Original language | English |
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Pages (from-to) | 1795-1800 |
Journal | Biotechnology Letters |
Volume | 25 |
DOIs | |
Publication status | Published - 1 Nov 2003 |
Keywords
- Fermentation
- Methanol concentration
- On-line monitoring
- Pichia pastoris
- Sequential injection analysis