On-line monitoring of the methanol concentration in Pichia pastoris cultures producing an heterologous lipase by sequential injection analysis

A. Surribas; O. Cos; J. L. Montesinos; F. Valero

doi:https://doi.org/10.1023/A:1026228518418

On-line monitoring of the methanol concentration in Pichia pastoris cultures producing an heterologous lipase by sequential injection analysis

A. Surribas, O. Cos, J. L. Montesinos, F. Valero

Research output: Contribution to journal › Article › Research › peer-review

24 Citations (Scopus)

Abstract

An automated sequential injection analysis (SIA) system using stop-flow technique was developed to determine methanol concentration by means of the enzymatic reactions of alcohol oxidase and peroxidase. Its application as an on-line device for monitoring Pichia pastoris fermentations producing an heterologous protein was demonstrated. Linear response, observed up to 2 g l-1, was reached by including a dilution chamber in the SIA manifold. The sampling frequency was 7 analyses per hour with a relative standard deviation lower than 4%.

Original language	English
Pages (from-to)	1795-1800
Journal	Biotechnology Letters
Volume	25
DOIs	https://doi.org/10.1023/A:1026228518418
Publication status	Published - 1 Nov 2003

Keywords

Fermentation
Methanol concentration
On-line monitoring
Pichia pastoris
Sequential injection analysis

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abstract = "An automated sequential injection analysis (SIA) system using stop-flow technique was developed to determine methanol concentration by means of the enzymatic reactions of alcohol oxidase and peroxidase. Its application as an on-line device for monitoring Pichia pastoris fermentations producing an heterologous protein was demonstrated. Linear response, observed up to 2 g l-1, was reached by including a dilution chamber in the SIA manifold. The sampling frequency was 7 analyses per hour with a relative standard deviation lower than 4%.",

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AU - Surribas, A.

AU - Cos, O.

AU - Montesinos, J. L.

AU - Valero, F.

PY - 2003/11/1

Y1 - 2003/11/1

N2 - An automated sequential injection analysis (SIA) system using stop-flow technique was developed to determine methanol concentration by means of the enzymatic reactions of alcohol oxidase and peroxidase. Its application as an on-line device for monitoring Pichia pastoris fermentations producing an heterologous protein was demonstrated. Linear response, observed up to 2 g l-1, was reached by including a dilution chamber in the SIA manifold. The sampling frequency was 7 analyses per hour with a relative standard deviation lower than 4%.

AB - An automated sequential injection analysis (SIA) system using stop-flow technique was developed to determine methanol concentration by means of the enzymatic reactions of alcohol oxidase and peroxidase. Its application as an on-line device for monitoring Pichia pastoris fermentations producing an heterologous protein was demonstrated. Linear response, observed up to 2 g l-1, was reached by including a dilution chamber in the SIA manifold. The sampling frequency was 7 analyses per hour with a relative standard deviation lower than 4%.

KW - Fermentation

KW - Methanol concentration

KW - On-line monitoring

KW - Pichia pastoris

KW - Sequential injection analysis

U2 - https://doi.org/10.1023/A:1026228518418

DO - https://doi.org/10.1023/A:1026228518418

M3 - Article

VL - 25

SP - 1795

EP - 1800

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

ER -