On-line monitoring of the methanol concentration in Pichia pastoris cultures producing an heterologous lipase by sequential injection analysis

A. Surribas, O. Cos, J. L. Montesinos, F. Valero

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    24 Citations (Scopus)

    Abstract

    An automated sequential injection analysis (SIA) system using stop-flow technique was developed to determine methanol concentration by means of the enzymatic reactions of alcohol oxidase and peroxidase. Its application as an on-line device for monitoring Pichia pastoris fermentations producing an heterologous protein was demonstrated. Linear response, observed up to 2 g l-1, was reached by including a dilution chamber in the SIA manifold. The sampling frequency was 7 analyses per hour with a relative standard deviation lower than 4%.
    Original languageEnglish
    Pages (from-to)1795-1800
    JournalBiotechnology Letters
    Volume25
    DOIs
    Publication statusPublished - 1 Nov 2003

    Keywords

    • Fermentation
    • Methanol concentration
    • On-line monitoring
    • Pichia pastoris
    • Sequential injection analysis

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