TY - JOUR
T1 - On-line monitoring of starch enzymatic hydrolysis by near-infrared spectroscopy
AU - Blanco, M.
AU - Coello, J.
AU - Iturriaga, H.
AU - Maspoch, S.
AU - González Bañó, R.
PY - 2000/1/1
Y1 - 2000/1/1
N2 - The enzymatic hydrolysis of starch is an industrial process involving two consecutive steps (viz., liquefaction and saccharification with the enzymes α-amylase and glucoamylase, respectively, which cleave α-1,4-glycosidic bonds and yield soluble dextrin chains in the first step and a mixture of glucose, maltose and residual higher polysaccharides in the second). This paper reports a near-infrared spectroscopic (NIRS) method for monitoring the process. Partial least-squares (PLS) regression is used to construct calibration models based on synthetic mixtures of sugars (glucose, maltose, dextrin and soluble starch) with a view to determining the glucose and polysaccharide concentrations, and the dextrose equivalent (DE). The models thus developed were used to monitor the degradation of starch from corn and dextrins; the results thus obtained were consistent with the DE provided by the Luff-Schoorl method. The proposed NIR method is thus an effective alternative to traditional methods for the same purpose.
AB - The enzymatic hydrolysis of starch is an industrial process involving two consecutive steps (viz., liquefaction and saccharification with the enzymes α-amylase and glucoamylase, respectively, which cleave α-1,4-glycosidic bonds and yield soluble dextrin chains in the first step and a mixture of glucose, maltose and residual higher polysaccharides in the second). This paper reports a near-infrared spectroscopic (NIRS) method for monitoring the process. Partial least-squares (PLS) regression is used to construct calibration models based on synthetic mixtures of sugars (glucose, maltose, dextrin and soluble starch) with a view to determining the glucose and polysaccharide concentrations, and the dextrose equivalent (DE). The models thus developed were used to monitor the degradation of starch from corn and dextrins; the results thus obtained were consistent with the DE provided by the Luff-Schoorl method. The proposed NIR method is thus an effective alternative to traditional methods for the same purpose.
U2 - https://doi.org/10.1039/a909248j
DO - https://doi.org/10.1039/a909248j
M3 - Article
VL - 125
SP - 749
EP - 752
ER -