An automated sequential injection analysis using stop-flow technique for the on-line determination of lipolytic activity has been developed. It is based on a colorimetric method using a chromogenic substrate, 1,2-O-dilaurylrac-glycero-3-glutaric acid-(6-methylresorufin)-ester. The system permits a linear range analysis between 5-100 lipolytic activity units m1-1, without external dilution of the sample, a sampling frequency of 5 samples per hour and a relative standard deviation (RSD) of 5%. The analyser has been used for the on-line monitoring of Candida rugosa fed-batch fermentation with excellent performance, regarding its reliability and reproducibility.
- Candida rugosa
- Lipolytic activity
- On-line monitoring
- Sequential injection analysis