On-line monitoring of lipolytic activity by sequential injection analysis

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    An automated sequential injection analysis using stop-flow technique for the on-line determination of lipolytic activity has been developed. It is based on a colorimetric method using a chromogenic substrate, 1,2-O-dilaurylrac-glycero-3-glutaric acid-(6-methylresorufin)-ester. The system permits a linear range analysis between 5-100 lipolytic activity units m1-1, without external dilution of the sample, a sampling frequency of 5 samples per hour and a relative standard deviation (RSD) of 5%. The analyser has been used for the on-line monitoring of Candida rugosa fed-batch fermentation with excellent performance, regarding its reliability and reproducibility.
    Original languageEnglish
    Pages (from-to)1783-1788
    JournalBiotechnology Letters
    Publication statusPublished - 12 Dec 2000


    • Candida rugosa
    • Fermentation
    • Lipolytic activity
    • On-line monitoring
    • Sequential injection analysis


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