On-line monitoring of lipolytic activity by sequential injection analysis

Oriol Cos; José Luis Montesinos; Javier Lafuente; Carles Solà; Francisco Valero

doi:https://doi.org/10.1023/A:1005654304541

On-line monitoring of lipolytic activity by sequential injection analysis

Oriol Cos, José Luis Montesinos, Javier Lafuente, Carles Solà, Francisco Valero

Research output: Contribution to journal › Article › Research › peer-review

8 Citations (Scopus)

Abstract

An automated sequential injection analysis using stop-flow technique for the on-line determination of lipolytic activity has been developed. It is based on a colorimetric method using a chromogenic substrate, 1,2-O-dilaurylrac-glycero-3-glutaric acid-(6-methylresorufin)-ester. The system permits a linear range analysis between 5-100 lipolytic activity units m1-1, without external dilution of the sample, a sampling frequency of 5 samples per hour and a relative standard deviation (RSD) of 5%. The analyser has been used for the on-line monitoring of Candida rugosa fed-batch fermentation with excellent performance, regarding its reliability and reproducibility.

Original language	English
Pages (from-to)	1783-1788
Journal	Biotechnology Letters
Volume	22
DOIs	https://doi.org/10.1023/A:1005654304541
Publication status	Published - 12 Dec 2000

Keywords

Candida rugosa
Fermentation
Lipolytic activity
On-line monitoring
Sequential injection analysis

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title = "On-line monitoring of lipolytic activity by sequential injection analysis",

abstract = "An automated sequential injection analysis using stop-flow technique for the on-line determination of lipolytic activity has been developed. It is based on a colorimetric method using a chromogenic substrate, 1,2-O-dilaurylrac-glycero-3-glutaric acid-(6-methylresorufin)-ester. The system permits a linear range analysis between 5-100 lipolytic activity units m1-1, without external dilution of the sample, a sampling frequency of 5 samples per hour and a relative standard deviation (RSD) of 5%. The analyser has been used for the on-line monitoring of Candida rugosa fed-batch fermentation with excellent performance, regarding its reliability and reproducibility.",

keywords = "Candida rugosa, Fermentation, Lipolytic activity, On-line monitoring, Sequential injection analysis",

author = "Oriol Cos and Montesinos, {Jos{\'e} Luis} and Javier Lafuente and Carles Sol{\`a} and Francisco Valero",

year = "2000",

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doi = "https://doi.org/10.1023/A:1005654304541",

language = "English",

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pages = "1783--1788",

journal = "Biotechnology Letters",

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TY - JOUR

T1 - On-line monitoring of lipolytic activity by sequential injection analysis

AU - Cos, Oriol

AU - Montesinos, José Luis

AU - Lafuente, Javier

AU - Solà, Carles

AU - Valero, Francisco

PY - 2000/12/12

Y1 - 2000/12/12

N2 - An automated sequential injection analysis using stop-flow technique for the on-line determination of lipolytic activity has been developed. It is based on a colorimetric method using a chromogenic substrate, 1,2-O-dilaurylrac-glycero-3-glutaric acid-(6-methylresorufin)-ester. The system permits a linear range analysis between 5-100 lipolytic activity units m1-1, without external dilution of the sample, a sampling frequency of 5 samples per hour and a relative standard deviation (RSD) of 5%. The analyser has been used for the on-line monitoring of Candida rugosa fed-batch fermentation with excellent performance, regarding its reliability and reproducibility.

AB - An automated sequential injection analysis using stop-flow technique for the on-line determination of lipolytic activity has been developed. It is based on a colorimetric method using a chromogenic substrate, 1,2-O-dilaurylrac-glycero-3-glutaric acid-(6-methylresorufin)-ester. The system permits a linear range analysis between 5-100 lipolytic activity units m1-1, without external dilution of the sample, a sampling frequency of 5 samples per hour and a relative standard deviation (RSD) of 5%. The analyser has been used for the on-line monitoring of Candida rugosa fed-batch fermentation with excellent performance, regarding its reliability and reproducibility.

KW - Candida rugosa

KW - Fermentation

KW - Lipolytic activity

KW - On-line monitoring

KW - Sequential injection analysis

U2 - https://doi.org/10.1023/A:1005654304541

DO - https://doi.org/10.1023/A:1005654304541

M3 - Article

VL - 22

SP - 1783

EP - 1788

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

ER -