On-line enzyme activity determination using the stopped-flow technique: Application to laccase activity in pulp mill waste-water treatment

X. Font; G. Caminal; X. Gabarrell; J. Lafuente; M. T. Vicent

doi:https://doi.org/10.1007/s002530051033

On-line enzyme activity determination using the stopped-flow technique: Application to laccase activity in pulp mill waste-water treatment

X. Font, G. Caminal, X. Gabarrell, J. Lafuente, M. T. Vicent

Research output: Contribution to journal › Article › Research › peer-review

8 Citations (Scopus)

Abstract

An automated system for on-line measurement of enzyme activity is proposed. The system uses a flow injection manifold in the stopped-flow mode to measure initial reaction rates. The time during which the flow is halted is selected in such a way as to optimise the enzyme/substrate ratio for the correct determination of activity values. The proposed system was used to determine the activity of laccase produced by the fungus Tranceres versicolor immobilised on nylon in a fixed-bed reactor used for treating pulp mill waste water.

Original language	English
Pages (from-to)	168-173
Journal	Applied Microbiology and Biotechnology
Volume	48
DOIs	https://doi.org/10.1007/s002530051033
Publication status	Published - 1 Jan 1997

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abstract = "An automated system for on-line measurement of enzyme activity is proposed. The system uses a flow injection manifold in the stopped-flow mode to measure initial reaction rates. The time during which the flow is halted is selected in such a way as to optimise the enzyme/substrate ratio for the correct determination of activity values. The proposed system was used to determine the activity of laccase produced by the fungus Tranceres versicolor immobilised on nylon in a fixed-bed reactor used for treating pulp mill waste water.",

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T1 - On-line enzyme activity determination using the stopped-flow technique: Application to laccase activity in pulp mill waste-water treatment

AU - Font, X.

AU - Caminal, G.

AU - Gabarrell, X.

AU - Lafuente, J.

AU - Vicent, M. T.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - An automated system for on-line measurement of enzyme activity is proposed. The system uses a flow injection manifold in the stopped-flow mode to measure initial reaction rates. The time during which the flow is halted is selected in such a way as to optimise the enzyme/substrate ratio for the correct determination of activity values. The proposed system was used to determine the activity of laccase produced by the fungus Tranceres versicolor immobilised on nylon in a fixed-bed reactor used for treating pulp mill waste water.

AB - An automated system for on-line measurement of enzyme activity is proposed. The system uses a flow injection manifold in the stopped-flow mode to measure initial reaction rates. The time during which the flow is halted is selected in such a way as to optimise the enzyme/substrate ratio for the correct determination of activity values. The proposed system was used to determine the activity of laccase produced by the fungus Tranceres versicolor immobilised on nylon in a fixed-bed reactor used for treating pulp mill waste water.

U2 - https://doi.org/10.1007/s002530051033

DO - https://doi.org/10.1007/s002530051033

M3 - Article

VL - 48

SP - 168

EP - 173

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

ER -