Abstract
An automated system for on-line measurement of enzyme activity is proposed. The system uses a flow injection manifold in the stopped-flow mode to measure initial reaction rates. The time during which the flow is halted is selected in such a way as to optimise the enzyme/substrate ratio for the correct determination of activity values. The proposed system was used to determine the activity of laccase produced by the fungus Tranceres versicolor immobilised on nylon in a fixed-bed reactor used for treating pulp mill waste water.
Original language | English |
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Pages (from-to) | 168-173 |
Journal | Applied Microbiology and Biotechnology |
Volume | 48 |
DOIs | |
Publication status | Published - 1 Jan 1997 |