On-chip magneto-immunoassay for Alzheimer's biomarker electrochemical detection by using quantum dots as labels

Mariana Medina-Sánchez, Sandrine Miserere, Eden Morales-Narváez, Arben Merkoçi

    Research output: Contribution to journalArticleResearchpeer-review

    63 Citations (Scopus)

    Abstract

    Electrochemical detection of cadmium-selenide/zinc-sulfide (CdSe@ZnS) quantum dots (QDs) as labeling carriers in an assay for apolipoprotein E (ApoE) detection has been evaluated. The immunocomplex was performed by using tosylactivated magnetic beads as preconcentration platform into a flexible hybrid polydimethylsiloxane (PDMS)-polycarbonate (PC) microfluidic chip with integrated screen printed electrodes (SPE). All the immunoassay was performed in chip and in flow mode. The sensitive electrochemical detection was obtained by square wave anodic stripping voltammetry. ApoE was evaluated for its potential as biomarker for Alzheimer's disease detection, achieving a limit of detection (LOD) of ~12.5ngmL-1 with a linear range from 10 to 200ngmL-1 and high accuracy for diluted human plasma. © 2013 Elsevier B.V.
    Original languageEnglish
    Pages (from-to)279-284
    JournalBiosensors and Bioelectronics
    Volume54
    DOIs
    Publication statusPublished - 15 Apr 2014

    Keywords

    • Alzheimer biomarker
    • Apolipoprotein E (ApoE)
    • Electrochemistry
    • Magnetic beads
    • Microfluidic
    • Quantum dots

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