Oligosaccharide structure determines prebiotic role of β-galactomannan against Salmonella enterica ser. Typhimurium in vitro

Roger Badia, Rosil Lizardo, Paz Martínez, Joaquim Brufau

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

Prebiotics and probiotics are considered natural alternatives to dietary antibiotics in animal production. Plant extracts and yeast cell walls are mannose-rich products that can be used as substrate for adhesion of Gram-negative bacteria. We assessed whether the structure of these saccharides is relevant to develop their role as prebiotics and therefore, their suitability to be used as alternatives to antibiotics to prevent intestinal infections in pigs. The prebiotic functionality of β-galactomannan (βGM), mannanoligosaccharide from yeast Saccharomyces cerevisiae (Mannan SC) and monosaccharide D-Mannose were studied in porcine intestinal epithelial cells (IPI-2I) challenged with Salmonella enterica ser. Typhimurium. Results showed that in vitro challenge with Salmonella induces the secretion of proinflammatory cytokine IL6 and chemokine CXCL8 compared with control without infection. Both βGM and Mannan SC, attenuate Salmonella-induced secretion of IL6 and CXCL8. Interestingly, cells treated with D-mannose showed similar levels of proinflammatory IL6 and CXCL8 compared with the control of infection. These data suggest that prebiotic role of βGM is related to its oligosaccharide structure. © 2013 Landes Bioscience.
Original languageEnglish
JournalGut Microbes
Volume4
Issue number1
DOIs
Publication statusPublished - 1 Jan 2013

Keywords

  • Intestinal epithelial cells
  • Mannanoligosaccharides
  • Saccharomyces cerevisiae
  • Salmonella Typhimurium

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