Occurrence of a LINE sequence in the 3′ UTR of the goat α<inf>s1</inf>-casein E-encoding allele associated with reduced protein synthesis level

Marta Jansà Pérez, Christine Leroux, Armand Sànchez Bonastre, Patrice Martin

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    102 Citations (Scopus)

    Abstract

    The E allele of the αs1-casein (αs1-Cas)-encoding gene is associated with a reduced casein content in milk. Structural analysis of mRNA and sequencing of amplified genomic DNA fragments, have revealed that this allele contains a 457-bp insertion within exon 19 (last untranslated exon). This insert is a truncated long interspersed repeated element (LINE) containing part of the ORF-2, the 3′ UTR and the poly (A) tail of the original retroposon. This LINE sequence was found to be highly repeated in the goat genome. The threefold reduction in the amount of αs1-CasE mRNA in the total, as well as in the polysomal fractions, as compared with its A counterpart, leads one to suppose either a reduced transcriptional rate of allele E or/and a decreased stability of the relevant mRNA. Northern blot and PCR experiments suggested the setting up of highly stable secondary structures involving the 3′ UTR of the αs1-CasE transcript. The mRNA folding calculations support such an hypothesis, by base-pairing interactions between the E allele messenger poly(A) tail and a long poly(U)-stretch occurring at the 5′ end of the insertion. In addition, since A + U-rich motifs are also present in the truncated LINE insertion, we propose that this extra sequence might be responsible for the lower milk αs1-Cas content by reducing the allele E mRNA stability. © 1994.
    Original languageEnglish
    Pages (from-to)179-187
    JournalGene
    Volume147
    Issue number2
    DOIs
    Publication statusPublished - 30 Sep 1994

    Keywords

    • goat
    • lactating mammary gland
    • Milk protein gene
    • mRNA stability
    • retroposon

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